中文摘要：

采用傅立叶变换红外光谱法并借助于二阶导数谱以及二维相关红外光谱（2D-FTIR）研究了蒙药草乌花原药材及不同溶剂提取物（依次采用乙醚,乙醇和水提取）所含化学成分的红外谱图整体变化规律。结果显示：在草乌花原药材中具有明显的多糖类化合物的红外特征吸收峰,证明该药材中含有大量多糖类成分。草乌花药材乙醚和醇提物的红外光谱较相似,有较强的羰基吸收峰（1734和1713 cm-1）和饱和烷基的特征吸收峰（2960,2920和2851 cm-1）,说明二者含有大量的脂肪酸（酯）等低极性成分。在分辨率较高的二阶导数谱中,两者的差异更为明显,水提物中含有脂肪酸（酯）类化合物,红外光谱法可以提供大量的结构信息。这种将药材整体谱图与不同溶剂提取物谱图及不同提取工艺之间的谱图相对照分析的研究方法,可观测药材化学成分的整体变化规律,有助于药材的整体质量控制、有效部位的定性宏观追踪及不同提取工艺的鉴别。

英文摘要：

Infrared spectrum changing rules of chemical components of Aconitum kusnezoffii and its extract by different solvents were studied using Fourier transform infrared spectroscopy,second derivative infrared spectroscopy and two dimensional infrared correlation spectroscopy.The results showed that there was abundance of polyose in the Aconitum kusnezoffii flowers,as the IR absorption peaks of polyose in the drug was observed.The IR spectra of ether and ethenol extract were similar,the strong absorption peaks of carbonyl and saturated alkyl groups could be detected,and this suggested that there were many low polarity chemical components like fatty acids.The differences between them were more obvious in the second derivative infrared spectroscopy.Fatty acids were detected in the water extract and structural information was obtained by analyzing the infrared spectroscopy.The method of analyzing the drugs by comparing the IR spectra of crude drugs,different solvents extractives and different extract process,could observe the changing rules of the chemical components of crude drugs.This method could be applied to the quality control,macrotrace and identification of extraction process.