中文摘要：

目的：引种、繁殖4.1R基因敲除小鼠,用于蛋白4.1R功能的研究。方法：按照国家进出境动物检验检疫的具体要求,对引种自美国的3对4.1R基因敲除小鼠进行隔离、检疫。引进的种鼠按照SPF动物饲养标准操作规程在IVC屏障系统中进行1雄1雌长期同居方式繁殖,C57BL/6J对照组小鼠采用同样方式饲养繁殖。定期观察记录种鼠的繁殖率、仔鼠出生率及成活率。比较基因敲除小鼠与野生型小鼠体质量变化。提取仔鼠尾组织基因组DNA,用PCR法进行基因型鉴定。结果：经隔离检疫,3对4.1R敲除小鼠符合我国SPF小鼠的微生物控制级别。23只繁殖仔鼠基因型PCR鉴定结果为基因敲除纯合子,雌鼠产仔率100%,平均胎产仔5.8只,仔鼠成活率74%。基因敲除小鼠平均体质量与野生型小鼠比较差异无统计学意义（F组间=4.035,P=0.056;F时间=543.723,P〈0.001;F交互=4.358,P=0.004）。结论：在国内成功引种和繁殖C57BL/6J-4.1R-/-小鼠。

英文摘要：

Aim：To introduce and reproduce 4.1R gene knock out mouse for exploring the functions of protien 4.1R.Methods：A total of 3 pairs of 4.1R gene knock out mice introduced from the USA were isolated and inspected according to the Animal Inspection and Quarantine Law of PR China.The introduced 4.1R knock out mice were breeding in IVC equipment according to the SPF Laboratory Animal Standard Operation Procedure.A pair of one male and one female homozygote were used to reproduce 4.1R knock out mice and C57BL/6J mice were used as control.The body weights and the rate of femal reproduction,birth and survival between gene konck out and wild type mice were recorded regularly for further analysis.The genomic DNA from the murine tail tissue was subjected to PCR for genotyping.Results：The inspection and quarantine results showed that the introduced 4.1R knock out mice were qualified for the SPF laboratory mouse grade of China.PCR results showed that 23 reproduced mice were 4.1R gene knock out homozygotes.The average litter size was 5.8 and the survival rate was 74%.Though no significant difference of weight was found between wild type and 4.1R knock out mice（Fgroups=4.035,P=0.056;Ftime=543.723,P0.001;Finter=4.358,P=0.004）.Conclusion：4.1R gene knock out mice has been introduced and reproduced successfully in China.