中文摘要：

以具有抗肿瘤抗菌活性的产孢子海洋真菌Aspergillus unguis DLEP2008001为出发菌株,对其采用介质阻挡放电等离子体技术进行了诱变处理.在采用肿瘤细胞替代模型——DNA损伤修复基因缺陷型的大肠杆菌E coli AB3027进行突变株活性筛选的过程中,比较了三种筛选方案,最终确定了一种使用96孔板液体摇床发酵的筛选方法,该方法与通常的液体深层发酵有较好的对应性,且方便易行.通过该方法,灵敏快捷的筛选出了基于DNA损伤机制的高活性突变株,同时也显示介质阻挡放电等离子体技术对提高该菌株生物活性具有显著效果.

英文摘要：

A marine sporogenous fungal strain Aspergillus unguis DLEP2008001 with anticancer and antibacterial activity was taken as a starting strain to be mutagenized by dielectric barrier discharge plasma technology （DBD）.A DNA damage repairing gene deficient E.coli strain AB3027 was used to screen the mutants of DLEP2008001.During the screening,three different screening methods were compared,and a method using shaking liquid fermentation in 96-well microtiter plates was determined to be the final solution.This method has good correlation with ordinary liquid submerged fermentation and convenience to be carried out.Using this screening method,several mutants with high activity were selected,based on DNA damage mechanism.Meanwhile,the results also exhibits the DBD plasma remarkable effect in improving this strain bioactivity.