中文摘要：

目的：研究间皮素（mesothelin,MSLN）基因被沉默后的卵巢癌细胞SKOV3-MSLN-shRNA增殖能力的变化,以及MSLN基因沉默对裸鼠卵巢癌腹腔移植瘤生长的影响。方法：利用针对MSLN基因的siRNA慢病毒液和对照组慢病毒液分别感染SKOV3细胞,建立SKOV3-MSLN-shRNA细胞和SKOV3-MSLN-neg细胞的稳定转染株;以SKOV3细胞作空白对照,用平板克隆形成法和细胞计数法检测3组细胞的增殖能力。应用3组细胞分别建立裸鼠卵巢癌腹腔移植瘤模型,2周后处死裸鼠,观察每组动物的成瘤率及每只裸鼠的肿瘤质量、肿瘤数目和肿瘤种植部位等。结果：SKOV3-MSLN-shRNA细胞的体外增殖能力明显低于SKOV3-MSLN-neg细胞和SKOV3细胞,差异有统计学意义（P〈0.05）。SKOV3-MSLN-shRNA组裸鼠成瘤率为60%,而2个对照组SKOV3-MSLN-neg和SKOV3细胞的裸鼠成瘤率均为100%,差异无统计学意义（P〉0.05）。SKOV3-MSLN-shRNA组动物的肿瘤数目、肿瘤质量及肿瘤种植部位明显低于2个对照组（P〈0.05）。结论：MSLN基因沉默可以降低卵巢癌SKOV3细胞的体外增殖能力,同时可以抑制卵巢癌裸鼠腹腔移植瘤的生长和种植。

英文摘要：

Objective：To study the influence of silencing mesothelin（MSLN） gene on the proliferation of ovarian cancer cell line SKOV3 in vitro the growth of human ovarian xenograft in nude mice in vivo.Methods：MSLN-silenced cells（SKOV3-MSLN-shRNA） and control cells（SKOV3-MSLN-neg） were established after being infected with RNA interference lentivirus and empty vector lentivirus,respectively.SKOV3 cells were used as blank control.Cell proliferation was assayed by clone formation test and cell counting assay.The xenografted model of the three cell lines were established in nude mice.After 2 weeks,the nude mice were sacrificed,and the tumor formation rate,tumor weight,tumor number,and tumor position were recorded.Results：The ability of proliferation of SKOV3-MSLN-shRNA cells was obviously decreased compared with SKOV3-MSLN-neg cells and SKOV3 cells in vitro.The difference was significant（P〈0.05）.In vivo,the tumor formation rate was 60% in xenografts of SKOV3-MSLN-shRNA cells and 100% in other two groups.The difference was not significant（P〉0.05）.The average tumor weight,tumor number,and number of tumor position were all decreased in SKOV3-MSLN-shRNA group compared with the two control groups（P〈0.05）.Conclusion：Silencing MSLN gene decreased the proliferation of ovarian cancer SKOV3 cells in vitro and inhibited the growth of xenografts of ovarian cancer cells in nude mice