中文摘要：

目的研究P13K／AKT信号转导通路对LPA保护顺铂诱导的卵巢癌SKOV3细胞凋亡作用的影响。方法舯法检测LPA和LY294002对DDP作用后的SKOV3细胞增殖活性的影响；Ho—echst33258荧光染色观察凋亡细胞；FCM分析细胞凋亡率；凝胶电泳观察凋亡细胞的DNA“梯状”条带；Westernblot检测LPA、LY294002对磷酸化Akt蛋白袁达的影响。结果LPA＋LY294002＋DDP组对SKOV3细胞增殖的抑制作用、凋亡小体的产生及细胞凋亡率高于LPA＋DDP组（P〈0．05），而与LY294002＋DDP组差异无统计学意义，DNA片段凝胶电泳示LPA作用后不产生明显的凋亡片段，LPA和LY294002同时作用可出现DNA断裂梯形条带。Westernblot结果示LPA作用后磷酸化Akt蛋白表达升高，而LY294002作用后，磷酸化Akt蛋白表达明显下降。结论LPA通过激活PBK／Akt信号转导通路抑制顺铂诱导的卵巢癌细胞的凋亡。

英文摘要：

Objective This study aims to investigate the relationship between PI3K/AKt signaling path~ way and the protection provided by LPA for cisplatin-induced apoptosis in ovarian carcinoma cells. Methods MTT assay was used to assess the proliferative activity of SKOV3 cells treated by DDP with or without LPA and LY294002. Hochest33258 was used to observe the apoptotic cells by fluorescence staining. FCM was used to detect the apoptosis of SKOV3 cells treated by DDP with LPA and LY294002. The specific DNA ＂ladder＂ pattern was used to confirm the apoptotic event. Western blotting was employed to detect the expression the levels of phosphorylated Akt protein. Results It revealed that LPA decreased the growth inhibition induced by DDP, but LPA ＋ LY294002 increased the growth inhibition induced by DDP. In addition, it was also found that the apoptosis percentage in the cells treated with LPA ＋ LY294002 ＋ DDP only exposed to LPA ＋ DDP. Neither the apoptotic body nor the specific DNA ＂ladder＂ pattern for apoptotic cells was found in the cells exposed to LPA, but they were seen in the cells which were exposed to LPA ＋ LY294002. Western blotting showed that LPA increased the expression level of expression of phosphorylated Akt protein, but LY294002 decreased. Conclusion LPA inhibit the apoptosis induced by DDP through the PI3K/AKt signaling pathway.