研究了载气体积流量对颗粒填充床内固态同步酶解乙醇发酵特性的影响。实验结果表明,随着载气体积流量的增加,基质表面生物膜内的乙醇浓度显著降低,包埋颗粒填充床乙醇发酵效率得到提高,但载气体积流量继续增加,则载气对生物膜产生强烈的剪切作用,引起生物膜部分脱落和生物膜内水分减少,导致填充床酶解和乙醇发酵的效率显著下降。在载气体积流量为30mL/min,最大纤维素消耗量为10.47g,得到最大乙醇平均得率0.02g/g纤维素基质,填充床反应器的孔隙率减小了31%。颗粒填充床有效的避免了基质坍塌现象,增强了载气在反应区域的流动过程并及时载出反应生成的乙醇,消除了乙醇对发酵过程的抑制作用;同时同步酶解发酵消除了葡萄糖对糖化过程的抑制作用。
Effect of flow rate of carrier gas on characteristics of ethanol production by simultaneous saccharification and fermentation in anaerobic packed bed with entrapped particles was investigated. The experimental results indicate that with the increase of the flow rate of carrier gas, the ethanol concentration in the yeast biofilm adsorbed on the surface of substrate particles obviously decreased, thus, the efficiencies of simultaneous saccharification and fermentation of the anaerobic packed bed were improved. However, with the further increase of the carrier gas flow rate, part of the biofilm fell off the surface of particles due to the strong shearing force from carrier gas, and the water content within these particles decreased. It resulted in a decrease in the efficiencies of enzymolysis and fermentation of cellulose in the packed bed. Furthermore, the maximal value of the consumed cellulose, 10.47 g , and the maximal average ethanol yield, O. 02 g/ (g substrate) , were respectively obtained, and the porosity of the packed bed decreased 31% at 30 mL/min the flow rate of carrier gas. The packed bed with entr the flow of carrier gas in ap th ped particles effectively avoids the matrix collapse phenomenon, enhances e reaction region, and then strips the ethanol out of the reactor in time, which decreases the inhibition of the ethanol on the fermentation. Also, the simultaneous saccharification and fermentation eliminate the inhibition of the glucose on the enzymolysis.