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中文摘要：
提出了一种新型的鼓泡式光生物制氢反应器,并将Rhodopseudomonas palustris CQK-01光合产氢菌在光生物制氢反应器中进行序批式培养,以葡萄糖为碳源底物,以590nm单波长光为光源,研究了不同鼓泡条件下光合细菌的生长、产氢及底物降解特性.实验结果表明,在光生物制氢反应器中适当鼓入氩气气泡可以有效降低反应器内液相氢浓度,减少产物反馈抑制作用,促进产氢性能的提高,并且不同鼓泡频率对光合细菌的生长和产氢有较大影响.实验中间隔3h鼓泡时光合细菌产氢性能最佳,反应器最大产氢量33.25mmol,光能转化效率为18.46%,产氢得率为1.79mol（H2）/mol（glucose）,平均底物消耗速率为0.28mmolL-1h-1和平均产氢速率为0.50mmolL-1h-1.
英文摘要：
Batch culture hydrogen production by the purple non-sulfur phototrophic bacteria Rhodoseudomonas palustris CQK-01 was experimentally investigated in a novel gas sparging photobioreactor. The effects of sparging frequency on the growth and hydrogen production performance of Rhodoseudomonas palustris CQK-01 are discussed. The experimental results showed that sparging Ar gas into the reactor effectively reduced hydrogen concentration in the culture and hence weakened the product feedback inhibition, resulting in enhancement of hydrogen production performance. The gas sparging frequency was found to be an important factor for bacterial growth and hydrogen production by photosynthetic bacteria（PSB）, and the optimum hydrogen production performance of the bioreactor was achieved under gas sparging with an interval of 3 h. The total hydrogen production, light conversion efficiency, hydrogen yield, average substrate consumption rate and average hydrogen production rate were 33.25 mmol, 18.46%, 1.79 mol （H2）/mol （glucose）, 0.28 mmol L-1 h-1 and 0.50 mmol L-1 h-1, respectively.
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