中文摘要：

为探讨内蒙古大青山土庄绣线菊根围丛枝菌根真菌（ arbuscular mycorrhizal fungi, AMF）多样性及季节变化规律,分别于2014年春、夏、秋3个季节采集土庄绣线菊根际土壤,采用湿筛倾析-蔗糖离心法分离AMF孢子,运用形态学方法进行种类鉴定。结果表明, AMF侵染强度、丛枝丰富度、孢子密度、种的丰富度的季节变化存在显著的差异,4个指标均显示夏季最高,秋季次之,春季最低。3个季节共分离出AMF10属44种,包括2个未知种,其中 Rhizophagus、 Funneliformis和Glomus为3个季节的共有优势属, Rhizophagus intraradices和Fun-neliformis mosseae为3个季节的共有优势种。丰富度、 Shannon指数和Simpson指数与全N和速效P呈显著负相关,与速效K呈显著正相关。丰富度与pH值呈显著负相关,与碱解N呈显著正相关。土庄绣线菊根际AMF季节变化与植物物候一致,并与土壤因子密切相关。

英文摘要：

In order to explore the arbuscular mycorrhizal fungi diversity and seasonal variation in rhizosphere of S. pubescens in Daqingshan Mountain, Inner Mongolia, China, rhizosphere soil samples were collected in spring, summer and autumn of 2014, respectively. AMF spores were separated with wet-sieving and decanting technique and identified by the morphological methods. The results indicated that the intensity of mycorrhizal colonization, the abundance of arbuscule, the spore density and the species abundance had significant differences between differ-ent seasons, and the maximum values of these four indexes were found in the summer, but the minimums were de-tected at spring. 44 AMF species belonging to 10 genera and 2 unidentified species were isolated from all soil sam-ples, the dominant genera of 3 seasons were Rhizophagus, Funneliformis and Glomus. R. intraradices and F. mosseae were the dominant species of 3 seasons. Abundance, Shannon index and Simpson index had significantly negative correlation with total Nitrogen and available Phosphorus, while they had were significantly positive correla-tion with available potassium. Abundance had negatively correlated with pH, and it had significantly positively correlated with alkaline Nitrogen. The seasonal variation of AMF in rhizosphere of S. pubescens was consistent with plant phenology, and closely related to soil factors.