中文摘要：

目的 探讨补阳还五汤对脑缺血后大鼠脑组织白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）表达的影响.方法 按随机数字表法将SD大鼠分为正常对照组、假手术组、模型组、补阳还五汤组,后3组再按给药后1、3和7 d分为亚组,每组5只.采用大脑中动脉闭塞法（MCAO）建立右侧局灶性脑缺血大鼠模型;补阳还五汤组于术后2 h起灌服补阳还五汤10 ml/kg（14.2 g/kg）、每日1次.分别于相应时间点处死大鼠后取脑组织,采用酶联免疫吸附法（ELISA）和逆转录-聚合酶链反应（RT-PCR）测定IL-1β和TNF-α的蛋白及mRNA表达.结果 正常对照组和假手术组有低水平的IL-1β和TNF-α蛋白及mRNA表达.模型组IL-1β、TNF-α蛋白及mRNA表达于给药后1 d开始升高,IL-1β蛋白及mRNA表达在3 d时达高峰后开始下降,TNF-α蛋白及mRNA表达于7 d达高峰.与模型组比较,补阳还五汤组给药后1、3和7 d脑组织中IL-1β和TNF-α蛋白及mRNA表达均明显下降[IL-1β蛋白（ng/L）,1 d：90.290±8.693比102.556±13.934,3 d：129.632±11.050比150.117±8.552,7 d：66.185±9.020比91.362±9.901;TNF-α蛋白（ng/L）,1 d：210.341±19.247比236.887±20.137,3 d：267.503±21.006比322.659±15.068,7 d：299.637±17.717比386.678±16.297;IL-1β mRNA,1 d：0.54±0.09比0.64±0.11,3 d：0.80±0.06比0.89±0.07,7 d：0.70±0.09比0.78±0.08;TNF-α mRNA,1 d：0.64±0.09比0.73±0.11,3 d：0.74±0.13比0.85±0.07,7 d：0.82±0.07比0.93±0.08],差异均有统计学意义（P＜0.05或P＜0.01）.结论 补阳还五汤可能通过下调缺血脑组织IL-1β和TNF-α的蛋白及mRNA表达来调控炎症因子的释放,从而起到脑保护作用.

英文摘要：

Objective To explore the effect of Buyang Huanwu decoction （BYHWD, 补阳还五汤） on pro-inflammatory cytokines in rats after focal cerebral infarction. Methods Adult Sprague Dawley （SD） rats were randomly divided into following groups： normal control, sham, model, BYHWD. The rats in latter three groups were subdivided into subgroups of 1, 3, and 7 days after medication, with 5 rats in each group. The right side focal cerebral infarction model was reproduced by middle cerebral artery occlusion （MCAO）.The rats in BYHWD group were gavaged with BYHWD of 10 ml/kg （14.2 g/kg, once a day） 2 hours after operation. Animals were sacrificed at corresponding time points. The protein and mRNA expression of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） were determined by enzyme linked immunosorbent assay （ELISA） and reverse transcription-polymerase chain reaction （RT-PCR）. Results There were low levels expression of IL-1β and TNF-α protein and mRNA in normal control group and the sham group. After cerebral infarction, the protein and mRNA expression of IL-1β and TNF-α began to increase in rats 1 day after the insult, and the protein and mRNA expression of IL-1β reached the peak on 3rd day, and then lowered, and the protein and mRNA expression of TNF-α reached the peak on 7th day.Compared with model group on 1st, 3rd and 7th day, the protein expression of IL-1β （ng/L： 90. 290±8. 693 vs. 102. 556±13. 934 on 1st day, 129. 632±11. 050 vs. 150. 117±8. 552 on 3rd day, 66. 185±9. 020 vs.91. 362 ± 9. 901 on 7th day） and TNF-α （ng/L： 210. 341 ± 19. 247 vs. 236. 887 ± 20. 137 on 1st day,267. 503±21. 006 vs. 322. 659± 15. 068 on 3rd day, 299. 637± 17. 717 vs. 386. 678± 16. 297 on 7th day）,and mRNA expression of IL-1β （1 day： 0. 54±0. 09 vs. 0. 64±0. 11, 3 days： 0. 80±0. 06 vs. 0. 89±0. 07,7 days： 0. 70±0. 09 vs. 0. 78±0. 08） and TNF-α （1 day： 0. 64±0. 09 vs. 0. 73±0. 11, 3 days： 0. 74±0. 13 vs. 0. 85± 0.07, 7 days： 0. 82±