中文摘要：

贴壁生长在微电极表面的细胞可引起贴壁电极界面阻抗的改变,从而可以获得细胞生理功能相关的生物信息。本研究采用微机械加工技术,在硅基底上设计了直径为20～50μm的20通道金微电极阵列（micro-electrode array,MEA）,用以构建能实时、连续、定量跟踪哺乳动物细胞形态和增殖分化改变的细胞阻抗测试传感器（electric cell-substrate impedance sensing,ECIS）,用于细胞与电极间的阻抗测试研究。通过对培养在微电极表面24h的成骨细胞Saos-2细胞系的阻抗谱测量发现,其阻抗值增加集中在中频10^2～10^4Hz之间,本结果符合细胞阻抗传感测量的理论模型分析。因此,该微电极阵列细胞阻抗传感器的研究,为进一步的细胞生理和药物分析等研究奠定了良好的基础。

英文摘要：

When adherently growing cells cultured on the surface of the micro-electrodes, impedance detection can provide information related to cellular physiological functions. To establish electric cell-substrate impedance sensing （ECIS） system for detecting of culture morphology and proliferation of cultured mammal cells in a real time, non-invasive and quantitative way, a cell based biosensor with 20 channel gold micro-electrode arrays （MEA） was designed in this study. Based on micromechanical systems, diameters of these electrodes were fabricated about 20-50 micron on silicon wafe. After human osteoblasts Saos-2 cell lines were cultured on the surface of the electrodes about 24 h later, the impedance increase was mainly focused on 10^2- 10^4 Hz of the frequency spectroscopy. The impedance detecting result was well according with analysis of the impedance models of the theoretic analysis. Therefore, this cell-based impedance biosensor with micro-electrodes can be used for studies of cell physiologies and drug screenings.