中文摘要：

目的 探讨三黄糖肾康颗粒对早期糖尿病肾病模型大鼠微炎症状态的影响。方法 研究时间为2012年8—10月,SPF级Wistar大鼠90只,雌雄各半,随机选取10只作为正常对照组,给予常规颗粒饲料喂养;其余80只随机分为模型组、西药组、中药预防组、中药低剂量组、中药高剂量组,各16只,均给予高脂高糖饲料喂养。1周后除正常对照组大鼠外其余各组大鼠均予以链脲佐菌素（STZ）溶液42 mg/kg一次性腹腔注射,诱导糖尿病模型,8只大鼠造模失败;余72只大鼠2周后收集24 h尿液检测尿微量清蛋白（MALB）以判定早期糖尿病肾病模型是否成功,其中6只大鼠糖尿病肾病模型造模失败。实验过程中,模型组3只大鼠死亡,西药组1只大鼠死亡,中药低剂量组、中药高剂量组各有2只大鼠死亡。正常对照组和模型组均给予蒸馏水灌胃,中药预防组大鼠自早期糖尿病肾病造模成功日起即开始给药,连续给药10周;其余各组造模成功2周后,分别给予相应药物灌胃,连续给药8周。西药组给予贝那普利灌胃,中药高剂量组使用浓度为3.84 g/ml的三黄糖肾康颗粒药液,中药预防组和中药低剂量组使用浓度为1.28 g/ml的三黄糖肾康颗粒药液。实验结束后测定大鼠血糖（FBG）、糖化血红蛋白（Hb A1c）、MALB、血清β2-微球蛋白（β2-MG）、血肌酐（Scr）、尿素氮（BUN）、血清及肾脏组织中C反应蛋白（CRP）,肾脏组织中核因子κB（NF-κB）、单核细胞趋化蛋白-1（MCP-1）、细胞表面趋化因子受体2（CCR2）mRNA及蛋白表达水平,光镜下观察肾脏组织形态变化（HE染色和PAS染色）。结果 模型组、西药组、中药预防组、中药低剂量组、中药高剂量组FBG、Hb A1c、MALB高于正常对照组（P〈0.01）;西药组、中药预防组、中药低剂量组、中药高剂量组FBG、Hb A1c、MALB、血清及肾脏组织中CRP,肾脏组织中NF-κB、MCP-1、CCR2 mRNA及蛋白表达水平?

英文摘要：

Objective To explore the effect of Sanhuangtangshenkang granule on the micro inflammatory state in rats with early diabetes nephropathy. Methods The study was conducted from August to October in 2012. The study animals were 90 grade SPF Wistar rats, female and male were half and half. 10 rats were randomly selected as normal control group and fed with conventional pellet feed. The remaining 80 rats were randomly divided into model group, western medicine group, traditional Chinese medicine prevention group, traditional Chinese medicine low dose group and traditional Chinese medicine high dose group. There were 16 rats in each group, and they were fed with high fat and high sugar diet. One week later, all the rats in each group were given intraperitoneal injection of streptozotocin （STZ） solution 42 mg/kg, except for the normal control group, in order to induce diabetic model. 8 rats failed to make the model ; the remaining 72 rats were collected 24 h urine after 2 weeks to detect urinary mieroalbumin （MALB） to determine whether the early diabetic nephropathy model was successful, among them 6 rats failed to make diabetic nephropathy model. In the course of the experiment, 3 rats in the model group died, 1 rat in the western medicine group died, 2 rats in the traditional Chinese medicine low dose group and high dose group of died, respectively. Both the normal control group and model group were given distilled water by gavage. The rats in traditional Chinese medicine prevention group began the administration at the beginning of the success of early diabetic nephropathy model, and continued for 10 weeks; the other groups were given corresponding medicine by gavage 2 weeks after the model success, and continued for 8 weeks. The western medicine group was given benazepril gavage, the traditional Chinese medicine high dose group used the liquid medicine of 3.84 g/ml Sanhuangtangshenkang concentration, the traditional Chinese medicine prevention group and the traditional Chinese medicine low dose group used the