中文摘要：

目的：研究内皮抑素和多西环素对黑色素瘤细胞的生长增殖及血管生成的影响方法：C57／BL6小鼠57只，进行小鼠B16黑色素瘤动物实验，将小鼠分多西环素组（D）、多西环素加内皮抑素组（DE）、内皮抑素组（E）和空白对照组（C）4组，给予内皮抑素和多西环素处理，取瘤组织进行苏本素-伊红（HE）染色，比较各组坏死率、微血管密度（MVD）的差异进行免疫组织化学染色检测肿瘤组织增殖细胞核抗原（PCNA）的表达结果：三个药物处理组MVD数量均较对照组少且差异有统计学意义（F=10．888，P〈0．05），表明多西环素和内皮抑素抑制肿瘤微血管生成：由于多西环素和内皮抑素减少了肿瘤血供进而抑制瘤细胞增殖、促进其坏死，三个处理组瘤细胞坏死率均较对照组高．其中多西环素加内皮抑素组（DE）与对照组间的差异有统计学意义（F=7．229，P〈0．05），PCNA在各处理组的表达较对照组低，差异均有统计学意义（F=17．729，P〈0．05）．结论：多西环素和内皮抑素联合使用，影响黑色素瘤组织PCNA的表达和微血管生成．明显抑制黑色素瘤细胞的生长增殖。

英文摘要：

Objective： To investigate the effect of endostatin and doxycyeline on melanoma cell proliferation and tumor angiogenesis. Methods： Murine B16 melanoma experiment was performed. The mice were divided into 4 groups： the endosiatin group（E group）, the doxycycline group（D group）, and group treated with endostatin and doxyeyeline（DE group）, and the control group with no treatment （C group）. HE staining was performed to compare the tumor necrosis rate and micro-vessel density （MVD） among these groups. Immunhistochemistry was employed to detect the expression of proliferating cell nuclear antigen（PCNA）. Results： The MVD in the three experimental groups were all less than in the control group（F= 10.888, P〈0.05）, indicating that doxyeyeline and endostatin inhibited tumor angiogenesis. By decreasing blood supply for tumor, doxycycline and endostatin inhibit tumor cell proliferation and promote tumor cell necrosis. Tumor cell necrosis rate in the three experimental groups were all higher than in the control group（F=7.229, P〈0.05）. PCNA expression in the three experimental groups were all statistically less than in the contuol group （F=17.729, P〈0.05）. Conclusion： The combination of endostatin and doxyeycline can influence PCNA expression and angiogenesis in melanoma, and can remarkably inhibit the melanoma cell proliferation.