中文摘要：

目的：探索外周血循环内皮细胞以及骨髓源性的间充质干细胞（MSC）在恶性黑色素瘤血管生成过程中的作用方法：将BrdU标记后的ECV304细胞接种至荷瘤的SCID小鼠体内，17d后处死小鼠，将肿瘤组织进行抗BrdU免疫组化染色；水囊引产的胎儿四肢骨的骨髓源性的MSC进行分选、扩增、验证。、PKH26染色后将MSC自鼠尾静脉接种至荷瘤（LiBr）的SCID小鼠体内，在荧光显微镜下观察肿瘤新生血管情况：将无PKH26染色的MSC自鼠尾静脉接种至荷瘤小鼠（B16）体内14d后处死动物，利用FISH技术和免疫荧光染色技术研究肿瘤组织血管生成情况。结果：BrdU免疫组化染色结果显示阳性的ECV304细胞可以作为血管内皮细胞参与黑色素瘤组织的血管生成；PKH26荧光染色的骨髓MSC在黑色素瘤组织内可以诱导分化、分裂增生参与肿瘤的血管形成：FISH和免疫荧光染色证实骨髓源性的MSC可以参与小鼠肿瘤新生血管形成、结论：在恶性肿瘤的生长过程中，不但肿瘤组织周围的血管内皮细胞通过出芽的形式参与肿瘤的血管生成．来自于骨髓的MSC和内皮细胞也可以参与肿瘤的血管生成、

英文摘要：

Objective： To investigate the function of peripheral blood circulating endotheliocytes and myelogenic mesenchymal stem cells （MSC） in the process of angiogenesis in melanoma. Methods： ECV304 endotheliocytes marked with BrdU were inoculated into the tail vein of SCID melanoma tumor-bearing mice and then anti-BrdU immunohistochemical staining was performed on sections of the tumor tissues. Myelogenic MSC in the bones of fetal mouse limbs retrieved through the induction of labor through artificial rupture of membranes were sorted by FACS and amplified in vitro. After PKH26 staining, the MSC were inoculated into the SCID LiBr melanoma tumor-bearing mice through the tail vein. New vessels in the tumor were observed using fluorescent microscopy. The MSC without PKH26 staining were inoculated into the SCID B16 melanoma tumor-bearing mice through the tail vein. The animals were sacrificed 14 days after inoculation and the angiogenesis in the tumor tissue was studied using fluorescent in situ hybridization （FISH） and immunofluorescence technologies. Results： The results of the BrdU immunohistochemical staining showed that the positive ECV304 cells functioned as vascular endothelial cells （VEC） in the process of angiogenesis in the tumor tissue. In the melanoma, the PKH26-stained MSC induced difterentiation and proliferation and then functioned in the tumor neovascularity. FISH and immunofluorescence staining showed that the myelogenic MSC could take part in the tumor neovascularity. Conclusions： In the process of malignant tumor growth, not only the VEC around the tumor can participate in the tumor neovascularity by pullulation, but the myelogenic MSC can also take part in the process of tumor angiogenesis.