中文摘要：

为建立可同时检测猪伪狂犬病毒（PRV）、猪圆环病毒2型（PCV2）与猪细小病毒（PPV）的三重PCR方法,根据Gen Bank登录的病毒相关基因序列,选择保守区域设计引物,经过反应条件的优化,建立了可同时检测以上3种病毒的PCR诊断方法,扩增的目的片段长度分别为192 bp（PRV）、255 bp（PCV2）和759 bp（PPV）。对PRV、PCV2和PPV的核酸检测最低浓度分别为：2.5×10^-2,2.2×10^-3和3.7×10^-2ng,证明该方法具有良好的特异性和较高的敏感性。应用该方法对56份临床样品进行检测发现,PRV、PCV2和PPV的阳性率分别为16.1%、50.0%和19.6%,二重感染率为12.5%,三重感染阳性率为3.6%。该方法的成功建立为快速高效地检测以上3种病毒提供了有效手段。

英文摘要：

A triple PCR method was established for simultaneous detection of pseudorabies virus （ PRV ）, porcine circovirus type 2 （PCV2） and porcine parvovirus （PPV） with a set of specific primers designed according to the published viral genome sequences in GenBank. The PCR products were 192 bp （PRV）, 255 bp （PCV2） and 759 bp （PPV）, respectively. The triple PCR method was demonstrated with good sensitivity and high specificity, and the detection limit of the three viruses were 2. 5×10^-2 ng, 2. 2×10^-3 ng and 3.7×10^-2 ng, respectively. Fifty-six clinical samples were detected by using this method. The results showed that the positive rates of PRV, PCV2 and PPV were 16. 1%, 50% and 19. 6% , respectively. The proportions of dual and triple infections were 12.5% and 3.6%, respectively. The triple PCR method established in this study is helpful for rapid and efficient detection of the three viruses.