本文以香港巨牡蛎(Crassostreahongkongensis)和太平洋牡蛎(C.gigas)为研究对象,进行牡蛎异源三倍体研究试验。在水温25℃条件下,香港巨牡蛎剥离卵子于海水中浸泡促熟1.5h后,与太平洋牡蛎精子受精,获得最高卵裂率为(24.40±3.79)%。受精后20min,采用盐度为10的低渗海水处理杂交受精卵25rain,可获得68.65%的异源三倍体幼虫,孵化率为12.71%。异源三倍体幼虫的存活率和生长率均未表现出优势。受精后第9天,异源三倍体幼虫的存活率仅为(O.116土0.023)%,平均壳长为(103.50±0.85)(m;对照组的存活率和壳长分别为(42.17士2.74)%和(123.25±8.60)μm。幼虫在附着变态前死亡。
Prior to the allotriploid induction, the feasibility of fertilization between Cras-sostrea hongkongensis (Lain and Morton, 2003) eggs and C. gigas (Thunberg, 1973) sperm had been verified before. Results showed that cleavage rate of (24.40±3.79)% of the inter-specific hybrid can be received with the eggs immersed in 25℃ seawater for 1.5h. The zy-gotes treated for 25 rain with a hypotonic solution of 10 salinities at 20rain after fertilization can yield the highest triploidy rate of 68.65% and the hatchery rate of 12.71%. No statisti-cal advantages of growth rate and survival rate were found in allotriploid group compared with control group. The survival rate and shell length of allotriploid group were (0. 116±0. 023)% and (103.50±0.85)ptm at the 9th day after and shell length of control group were (42.17±2. riploid hybrid larvae died before metamorphism. insemination. However, the survival rate 74)% and (123.25±8.60)ttm. All allot-