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中文摘要：
精氨酸是仔猪生长必需的氨基酸之一，精氨酸不足可能是限制哺乳仔猪获得最佳生长的一个主要因素。选用12头21日龄体重相近的三元杂交（杜×长×大）仔猪，随机分为2组，每组设6个重复。试验组13粮中添加6．0g／kg的精氨酸饲喂至28日龄，对照组日粮中添加12．3g/kg的丙氨酸饲喂至28日龄，试验期为7d。收集并筛选仔猪空肠粘膜上皮组织样品，然后通过蛋白质双向电泳筛选缓解断奶应激的相关蛋白，共检测到8个差异蛋白点。其中，与对照组相比，2个差异蛋白上调，6个差异蛋白下调。上调的蛋白包括甲状腺激素受体结合因子10、谷胱甘肽硫转移酶、电子转移黄紊蛋白a亚基前体等，下调的蛋白包括异柠檬酸脱氢酶、线粒体ATP合酶、H’转运复合体F1B亚基、肽酰脯氨酰异构酶B前体等。这有助于探讨精氨酸在缓解仔猪断奶应激中的作用机理。
英文摘要：
Dietary arginine supplementation enhances the immune status and protein synthesis in early-weaned pigs. However, the underlying mechanisms remain largely unknown. To investigate how arginine affects the expression of key proteins that regulate growth and nutrient transport of jejunum, a total of 12 healthy piglets （21 day-old, similar body weight, Landrace x Yorkshire） delivered by four sows were randomly divided into two groups. Piglets in the test group were reared with feed supplemented L-arginine at a concentration of 6.0 g/kg, while piglets in the control group were fed with feed supplemented L-alanine at a concentration of 12.3 g/kg （isonitrogenous control）. After 7 d, jejunum mucosae was collected and analyzed with the 2-D PAGE MS technology. Compared with the control pigs, arginine decreased the levels of proteins that regulate the protein syn- thesis, intermediary metabolism and tissue growth （ similar to anterior gradient 2 homolog, cyclophilin_ABHJike, hypothetical protein FLJ39502 and tetratrioopeptide repeat domain 16, similar to KIAA0156, mitechondrial ATP synthase, hydrion transporting F1 complex, beta subunit and alpha-tu- bulin ubiquitous isoform 19, prolyl 4-hydroxylase, beta subunit precursor）. In addition, arginine increased the levels of proteins that are involved in proteolysis and immune response （ PGAM1, T cell receptor beta variable 20, membrane steroid binding protein, similar to myomesin-1, and chain A, structure of pig muscle Pgk complexed with MgATP）. Therefore, arginine influences the immune response and protein synthesis mechanisms as well as improves eady weaned stress syndrome of piglets.
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