利用组织分离法从党参中分离得到32种内生菌,其中菌株D19转化人参主皂苷为稀有皂苷的活性最强.在最佳发酵条件(水培养基,pH 5.0)下,稀有人参皂苷F2、C-K和Rh1的最高产率分别为30%、17%和8%.根据单体皂苷转化实验确定了稀有人参皂苷的来源和人参皂苷Rb1的转化路径,并通过16SrDNA序列分析,鉴定内生菌D19为藤黄色杆菌属(Dyella).
A total of 32 kinds of endophytes form Codonopsis pilosula were isolated through the method of organizational separation.Among these endophytes,strains D19 showed the strongest activities to convert major ginsenosides to minor ginsenosides.When in the medium of H2 O,pH of 5.0,the maximum yield of minor ginsenosides F2,C-K and Rh1 reached to 30%,17% and 8%,respectively.The source of minor ginsenosides and the transformation pathway of ginsenoside Rb1 were confirmed according to the saponin mon-omer conversion experiments.Phylogenetic analysis based on 16S rDNA sequences indicated that the strain D19 belongs to the genus Dyella.