中文摘要：

以紫外线辐射的葡萄幼果为材料提取总RNA,用SMART^TM cDNA文库构建试剂盒构建cDNA文库,初始文库含有1.5×10^6个独立的单克隆,扩增后滴度达到1.2×10^8pfu·mL^-1.随机挑取噬菌斑进行PCR检测,发现重组率在92.9%以上.

英文摘要：

The total RNA was isolated from young fruits of grape challenged with ultraviolet radiation,and with the isolated total RNA,a cDNA library with more than 1.5 ×10^6 individual clones was constructed with smarttm cDNA construction kit.The titer of the amplification cDNA library was more than 1.2×10^8 pfu·mL^-1 and the recombinant ratio was more than 92.9%.