中文摘要：

目的检测白细胞介素-6（IL-6）在人脑胶质瘤干细胞（GSCs）中的表达及其对GSCs侵袭力的影响。方法从8例人脑胶质瘤中培养出GSCs并予以鉴定。实时定量RT—PCR及酶联免疫吸附试验法检测IL-6在GSCs以及相应原代培养胶质瘤细胞（PGCs）中的表达水平并比较分析；使用中和性IL-6抗体或外源性IL-6后进行基质胶侵袭实验，检测GSCs及相应PGCs侵袭力的变化并比较分析。结果配对t检验结果显示IL-6在GSCs中mRNA和蛋白表达水平均显著高于相应的PGCs（分别为t=2．578，v=7，P〈0．05和t=4．690，v=7，P〈0．005）；基质胶侵袭实验中，加入IL-6抗体可以明显减低PGCs和GSCs的侵袭力（分别为t=3．105，v=7，P〈0．05和t=13．922，v=7，P〈0．001）；而外源性IL-6则可增加PGCs和GSCs的侵袭力（分别为t=-5．983，v=7，P〈0．001和t=-6．935，v=7，P〈0．001）。结论IL-6在GSCs中高表达，并且在体外实验中与PGCs和GSCs的侵袭性相关。IL-6可能在胶质瘤免疫抑制及肿瘤侵袭中起关键作用。

英文摘要：

Objective To investigate the expression of interleukin-6 in glioma stem cells （GSCs） and its role in glioma stem cells invasion. Method GSCs from eight surgical glioma specimens were cultured and identified. Real-time RT-PCR and Enzyme-Linked Immunosorbent Assay were used to measure and compare the expression levels of IL-6 in GSCs and corresponding primary glioma cells （PGCs）. Subsequently, IL-6 antibody or exogenous IL-6 was used in a Matrigel-invasion assay and effects of IL-6 on GSCs or PGCs invasiveness were then determined. Results Paired t-test showed that IL-6 mRNA and protein expression levels were significantly higher in GSCs than in PGCs from the identical tumor （ t =2.578,v =7, P 〈0.05 and t =4.690, t, =7, P 〈 0. 005, respectively）. In Matrigel-invasion assay, PGCs and GSCs invasiveness markedly decreased after IL-6 was blocked with neutralizing antibody （t =3.105, v =7, P 〈0.05 and t = 13. 922, v =7, P 〈0.001, respectively）, but significantly increased when exogenous IL-6 was added （ t = -5. 983, v =7, P 〈0. 001 and t = -6. 935, v = 7, P 〈 0. 001, respectively）. Conclusions The present study found that the IL-6 is overexpressed in GSCs and significantly augments GSCs invasiveness in vitro, suggesting a potential target in future therapy for GSCs rather than for their derivatives.