中文摘要：

目的：构建含有人Annexin A2基因的真核表达质粒pIRES2-eGFP-Annexin A2，并在真核细胞293T中表达。方法：将质粒pCMV5-Annexin A2上Annexin A2基因酶切后与真核表达质粒pIRES2-eGFP连接，酶切鉴定及测序正确后，脂质体介导法转染293T细胞，荧光定量PCR及蛋白质印迹法检测其基因和蛋白的表达。结果：构建的质粒pIRES2-eGFP-Annexin A2转染293T细胞后，目的基因mRNA和蛋白表达均明显增高。结论：成功构建pIRES2-eGFP-Annexin A2质粒并表达蛋白，为研究Annexin A2的生物学功能奠定了基础。

英文摘要：

Objective： To construct pIRES2-eGFP-Annexin A2 eukaryotic expression vector carrying human Annexin A2 gene and express Annexin A2 protein in 293T cell.Methods： Annexin A2 gene digested from pCMV5-Annexin A2 was ligated into eukaryotic expression vector pIRES2-eGFP.After restriction analysis and sequencing,the recombinant plasmid pIRES2-eGFP-Annexin A2 was transfected into 293T cells in the mediation of liposome.The expression of Annexin A2 was analyzed by western blot and real-time PCR. Results： The expression of Annexin A2 at both mRNA and protein levels were significantly increased when transfected with pIRES2-eGFP-Annexin A2 in 293T cells. Conclusion： The eukaryotic expression vector pIRES2-eGFP-Annexin A2 was correctly constructed and the Annexin A2 protein was successfully expressed in 293T cells.This will facilitate the following study on Annexin A2.