中文摘要：

目的制备兔抗人β2糖蛋白Ⅰ多克隆抗体，并鉴定抗体的特异性及功能活性。方法以纯化的人血浆p2糖蛋白Ⅰ（β2-GPI）免疫新西兰大白兔，获得抗血清；利用ProteinA及β2-GPI亲和层析柱纯化血清中总IgG和抗β2-GPI多克隆抗体；用免疫双向扩散试验（IDD）、ELISA和western-blot等方法鉴定抗体。结果IDD及ELISA鉴定抗体效价分别达到16和1×10^5；western-blot显示纯化的总IsG和抗β2-GPI抗体与β2-GPI蛋白均有特异反应条带；且抗体与β2-GPI复合物能够刺激单核细胞株THP-1表达组织因子（TF）活性。结论成功获得并纯化兔抗人β2-GPI多克隆抗体，为进一步研究β2-GPI／抗β2-GPI复合物在抗磷脂抗体综合征血栓形成机制中的作用建立了基础。

英文摘要：

Objective To prepare rabbit polyclonal antibody against human β2-GPI and to characterize the specificity and biological functions of the antibocly. Methods A rabbit was immunized with purified-human β2-GPI- The serum whole IgG and anti-β2-GPI antibody were purified by protein A and β2-GPI affinity chromatography respectively. The specificity of the antibody was determined by IDD, ELISA, and Western blotting （ WB）, and the function of the antibody was analyzed. Results The reactive titers of the anti-serum and the whole IgG reached to 1：16 and 1：100 000 with IDD and ELISA respectively. Both the whole IgG and anti-β2-GPI antibody reacted with β2-GPI in WB. The complex of the antibody and β2-GPI induced the monocytic cell line THP-1 to express tissue factor. Conclusions The polyclonal antibody against human β2-GPI has been prepared in the present study, which provides an efficient reagent for studying mechanisms of the thrombosis associated with antiphospholipid syndrome.