中文摘要：

目的：研究紫草效应成分动态变化因素,明晰紫草渗漉液浓缩过程中紫草素类成分的巨大损失原因。方法：采用动态取样,以HPLC法检测,考查5个紫草素类成分及相关因素在紫草渗漉液50℃回收乙醇时的动态变化,据此选择并考查3个关键因素温度、pH值及药液含醇量在单因素及多因素综合作用下对5个紫草素类成分的化学稳定性影响。结果：紫草渗漉液在50℃回收乙醇过程中,药液体积、含醇量不断减少的同时,pH值由5.56降至4.5左右,5个紫草素类成分的含量亦显著降低。关键因素药液温度、pH值及含醇量综合作用结果表明紫草渗漉液中5个紫草素类成分在40~60℃、pH 3~7、药液含醇量35%~70%时6 h内稳定。但药液含醇量降低时,紫草素类成分易析出,样品处理方式不当导致测定结果偏低,从而产生＂损失＂现象。结论：研究揭示紫草渗漉液浓缩过程中紫草素类成分的巨大＂损失＂真正原因并非是其化学稳定性差发生了降解转化,而是因其强脂溶性紫草素类成分不断析出,使溶液中含量显著降低所致。

英文摘要：

Objective：By studying on the factors influencing the dynamic changes of shikonin and its derivant,to interpret the great loss of these components in concentrating process of percolate of Arnerbia euchroma.Methods：By sampling dynamically,and using HPLC for detection,dynamic changes of 5 components of shikonin and its derivant and relative factors were investigated during concentrating process at 50℃,and influence of three key factors including temperature,pH and ethanol content on these components′ stability were further investigated through single factor and multiple factors tests.Results：The content of 5 major components lost greatly during concentrating process,with volume and ethanol content decrease continually and pH from 5.56 to 4.5.Single factor and multiple factors tests about three key factors including temperature,pH and ethanol content indicated that these 5 components remained stable for 6 hours under condition of 40~60 ℃,pH 3~7 and ethanol content 35%~70%.However,these components will precipitatie when the ethanol content of percolate became very low,which led to much lower content results and ＂loss phenomenon＂ of shikonin and its derivant.Conclusion：The true causes for ＂great loss＂ of shikonin and its derivant during concentrating process do not lie in their chemical transformation due to poor stability,but lie in continual precipitation of these liposoluble components during concentrating process,which leads to content decrease significantly.