欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的：利用泼尼松龙诱导的斑马鱼骨质疏松模型筛选续断抗骨质疏松活性部位,探讨斑马鱼骨质疏松模型的适用性与合理性。方法：将受精后5 d的斑马鱼幼鱼暴露在25μmol/L泼尼松龙溶液、0.5%DMSO的培养基中,48 h后将25μmol/L泼尼松龙溶液更换为含25μmol/L泼尼松龙的不同浓度续断总提物及其大孔树脂不同浓度醇洗脱部位（含生药0.025、0.25、2.5、25μg/mL）溶液中,同时保留一组25μmol/L泼尼松龙组作为模型对照组。28.5℃下在24孔板中培养,每天换液至第10 d处死。采用茜素红对培养10天的各组斑马鱼幼鱼骨骼染色,并以显微检测、数码成像方法定量分析骨骼染色区域。结果：与DMSO溶媒阴性对照组比较,25μmol/L泼尼松龙模型组的斑马鱼头部骨骼染色面积和染色光密度值显著减少;与模型组比较,除续断大孔树脂水与10%乙醇洗脱部位外,续断总提物及其大孔树脂30%、50%、70%、90%乙醇洗脱部位的斑马鱼头部骨骼染色面积和染色光密度值显著增加。色谱分析表明30%、50%、70%、90%乙醇洗脱部位即含皂苷类成分又含非皂苷类成分。结论：续断皂苷类和非皂苷类成分均能阻止泼尼松龙诱导的斑马鱼骨丢失。斑马鱼骨质疏松模型成功用于高效筛选中药续断的抗骨质疏松活性部位,具简单、高效及可操作性强的优势。
英文摘要：
Objective： Prednisolone-induced osteoporosis model using zebrafish was used to screen the antiosteoporotic active parts of Dipsacus Radix,in order to investigate the applicability and rationality of the zebrafish model of osteoporosis.Methods： Zebrafish larvae at 5 days post fertilization（ dpf） were exposed with 25 μmol /L prednisolone and 0.5% DMSO for 48 h,then except one group of 25μmol/L prednisolone,other groups of 25 μmol/L prednisolone were treated with a range of concentration（ 0.025,0.25,2.5,25 μg crude drug /mL） of extract of Dipsacus Radix and its different concentration ethanol elution parts of macroporous resin with 25 μmol /L prednisolone.All groups were incubated in 24-well plates（ 28.5 ℃） until 10 dpf.Zebrafish skeleton at 10 dpf were anesthetized and fixed for staining with alizarin red.Quantitative analysis of the stained area was performed by microscopic inspection and digital imaging methods to reflect the amount of zebrafish head skeleton mineralization.Results： The results indicated that head skeleton mineral area and integrated optical density（ IOD） of 25 μmol /L prednisolone model group were significantly decreased when compared with vehicle control group,and the extract of Dipsacus Radix and its 30%,50%,70% and 90% ethanol elution parts of macroporous resin rescued the further bone loss of zebrafish induced by prednisolone when compared with the model group.HPLC analysis indicated that components of 30%,50%,70% and 90% ethanol elution parts of macroporous resin containing saponins and nonsaponins components.Conclusion： Both saponins and nonsaponins can prevent bone loss of zebrafish induced by prednisolone.This novel osteoporosis zebrafish model was successfully used to screen antiosteoporotic active parts of Dipsacus Radix,which had advantages of simple,high efficiency and easy to perform.
同期刊论文项目
同项目期刊论文
Revolving Door Action of BCRP Facilitates or Controls the Efflux of Flavone Glucuronides from UGT1A9
Metabolism Study of Notoginsenoside R(1), Ginsenoside Rg(1) and Ginsenoside Rb(1) of Radix Panax Not
Metabolite Profiles of Epimedin B in rats by Ultra-performance Liquid Chromatography/quadrupole-time
期刊信息
位置：