中文摘要：

利用pFGC5941构建了甘蓝型油菜3个AP3重复基因的植物反义表达载体pF—GC5941-BnAP3—2、pFGC5941-BnAP3—3和pFGC5941-BnAP3—4．采用快速冻融法，将载体导入农杆菌EHA105，转化甘蓝型油菜下胚轴．在5mg／LPPT的MS培养基中筛选，获得反义BnAP3—2基因的抗性再生植株31株，反义BnAP3—3基因的抗性再生植株20株，反义BnAP3—4基因的抗性再生植株42株．PCR鉴定结果显示，获得BnAP3—2反义的转基因植株12株，BnAP3—3反义的转基因植株7株，BnAP3—4反义的转基因植株16株．半定量PCR分析结果显示，三个外源AP3基因的反义链已在油菜叶片中表达．

英文摘要：

Three AP3 homologous sequences,BnAP3- 2, BnAP3- 3 and BnAP3- 4, were reverse inserted into plasmid pFGC5941, constituted the anti-expression vectors pFGC5941-BnAP3-2, pFGC5941- BnAP3-3 and pFGC5941-BnAP3-4. The vectors were introduced into the grobacteriun strain EHA105 by means of rapid frozen thaw method and transferred into hypocotyls of Brassica napus. Thirty one re- sistant plants with antisense BnAP3-2 gene, twenty resistant plants with antisense BnAP3-3 gene and forty two resistant plants with antisense BnAP3-4 gene were obtained from the MS medium with 5 mg/L PPT. Twelve transgenic plants with antisense BnAP3-2 gene, seven transgenic plants with antisense BnAP3-3 gene and sixteen transgenic plants with antisense BnAP3-4 gene were identified by PCR. Semi-quantitative PCR analysis confirmed that antisense fragment of the there foreign AP3 homologous genes had been expressed in the B. napus plants.