中文摘要：

为克隆筛选棉花腺体形成相关的基因，运用SMART技术构建eDNA文库．首先抽提棉花腺体形成时期的mRNA，mRNA逆转录后合成eDNA，经均一化处理后，Sfi I酶切，连接质粒载体，电转化，成功构建了棉花腺体形成时期的eDNA文库，经鉴定原始文库滴度为5．8×10^5cfu／mL，其重组率高达94％，插入片段的平均长度约为1．4kb．

英文摘要：

A Normalized cDNA library was constructed from upland cotton during its pigments gland forming age with SMART（switching mechanism at 5 end of RNA transcript） technique using Creator SMART cDNA library construction kit. mRNAs were isolated from upland cotton using Reagent; Double-stranded cDNAs were synthesized from mRNAs and were processed by normalization and Sfi I and then the cDNAs were ligated to pDNR-LIB vector. The ligation mixture was transformed into E. coli JM109 by electroporation. Counting the number of colonies, the titer was 5.86 × 10^5 cfu/mL in this library. Electrophoresis gel results fragments ranged from 800 bp to 2 kb, with an average size of 1400 bp. Random picking clones showed that the recombination rate was 94 %. Our successfully constructed cDNA library is a full-length library with high quality, and can be used to screen the genes related to development of pigments gland cottons.