中文摘要：

以茎瘤芥（Brassica juncea var. tumida Tsen et Lee）‘永安’为材料，通过 RACE 和 RT-PCR技术得到 1 个 AP2/EREBP 转录因子基因的 cDNA 全长序列和基因组 DNA（gDNA）序列，该基因与拟南芥AtABR1基因的氨基酸序列相似性高达72%，因此命名为BjABR（1GenBank登录号：JQ713825.1）。BjABR1基因 gDNA 序列含 1 个内含子；cDNA 序列全长 1 514 bp，含有 1 个 1 146 bp 的开放阅读框（ORF），编码 381 个氨基酸；其推定编码的蛋白分子量为 41.674 kD，等电点为 9.11，具有 14 个磷酸化位点，含 1个典型的 AP2 DNA 结合域和 1 个 CMX-1 基序。洋葱表皮细胞的瞬时表达显示，BjABR1 蛋白定位于细胞核。荧光定量 PCR 分析结果表明，该基因在茎瘤芥不同发育时期的根、茎、叶中均有表达，但在根中表达量极高；在对茎瘤芥组培幼苗进行高盐、渗透压和低温 3 种非生物胁迫处理后发现，3 种胁迫均能诱导该基因表达，但其对高盐的响应更为迅速。

英文摘要：

The full length eDNA sequence and genomic DNA （gDNA） sequence of an AP2/EREBP transcription factor family gene were cloned from Brassicajuncea var. tumida Tsen et Lee ＇Yong＇an＇ by RACE （rapid amplification of cDNA ends） and RT-PCR. Amino acid sequence alignment showed the gene shared 72% similarity with a known Arabidopsis thaliana AP2/EREBP family gene, AtABR1, named after BjABR1 （GenBank accession No. JQ713825.1 ）. BjABR1 gene contained one intron and putatively encoded 381 amino acids with the protein molecular mass of 41.674 kD, the pI of9.11, 14 phosphorylation sites and an AP2 DNA-bind domain and a CMX-1 motif. Subcellular localization assays showed that theBjABR1 protein appeared in the nucleus. Quantitative real-time PCR analysis revealed that BjABR1 gene was expressed in root, stem and leaf, and highest in the root. The expression of BjABR1 was inducible under salt stress, osmotic stress and cold stress, and its transcriptional responses subject to salinity was most sensitive.