中文摘要：

以茎瘤芥幼苗叶片总RNA为模板，通过SMARTRACE技术进行反转录和PCR扩增．克隆到瘤芥酸性核糖体蛋白P1基因（命名为BjARPPl，GenBank登录号：JX282179），并利用ProtParam、DNAMAN等生物信息学工具对其核酸序列和蛋白序列进行分析。结果表明，茎瘤芥酸性核糖体蛋白PI基因开放阅读框为342bp。编码113个氨基酸。等电点为4．27，相对分子质量为11．25ku；二级结构预测显示α-螺旋占53．98％、不规则卷曲占33．68％、延伸链占12139％，还含有5个磷酸化位点：同源进化树预测分析其与拟南芥的同源性为96％，与其他物种同源性低。茎瘤芥酸性核糖体蛋白P1基因为首次克隆，为进一步研究该基因的结构、功能、遗传变异规律以及其与生产性能的关系提供科学依据。

英文摘要：

Total RNA from Brassicajuncea＇s leaf blades were used as the template to clone the cDNA sequence of acidic ribosomal protein P1 gene （named BjARPP1, GenBank NO ：JX282179）by SMART RACE technology and PCR. Then, bioinformatics softwares were used to analysis the nucleic acid sequence and the protein sequence.The results showed that the largest open reading frame of BjARPP1 had 342 bp in length and encoded a polypeptide of 113 amino acid residues. The estimated molecular weight and isoelectric point of the putative protein were 11.25 ku and 4.27, respectively. The predicted secondary structure showed that the protein contained 5 phosphorylation sites and had about 52.98% alpha helixes, 33.687% random coil and 12.29% extended strand. Its homology with Arobidopsis was as high as 96%, but the homology with other plants was low. The acidic ribosomal protein P1 gene of Brassicajuncea was cloned for the first time, which could provide scientific evidence for the study on its structure, functioning and genetic variations and its relationship with production performance.