中文摘要：

目的：探讨人类肺癌细胞株中,不同剂量的多西他赛对真核翻译起始因子3a（eukaryotic in-itiation factor 3 subunit a,eIF3a）和α-微管蛋白（α-tubulin）表达的影响及其时效、量效关系。方法：人类肺腺癌细胞株A549用不同浓度的多西他赛处理不同的时间后,Real-time PCR法检测α-微管蛋白和eIF3a的mRNA表达水平,Western印迹法检测α-微管蛋白和eIF3a的蛋白表达水平。结果：多西他赛不能影响α-微管蛋白mRNA和蛋白水平的表达,且eIF3a与α-微管蛋白的表达水平之间没有明显的相关性。高浓度（30μg/L）多西他赛处理细胞后,eIF3a的mRNA表达水平随着时间的延长有上升的趋势。结论：多西他赛在高剂量时具有增加eIF3amRNA表达的趋势,eIF3a不参与α-微管蛋白表达的调节。

英文摘要：

Objective To explore the dose-dependent and time-dependent effect of docetaxel on the expression of mammalian eukaryotic initiation factor 3 subunit A（eIF3a） in lung cancer cell line.Methods The human lung cancer cell line A549 was treated with gradient concentrations of docetaxel for different time.Real-time PCR and Western blot were used to detect mRNA and protein expression levels of eIF3a and α-tubulin,respectively.Results Docetaxel did not affect α-tubulin expression at either mRNA level or protein level.When A549 cells were treated with high concentration of docetaxel（30 μg/L）,the expression level of eIF3a mRNA tended to increase in a time-dependent manner.Protein expression level of α-tubulin was not associated with eIF3a expression significantly in cells treated by docetaxel.Conclusion Docetaxel could slightly increase the expression of eIF3a mRNA,and eIF3a does not regulate the expression of α-tubulin in A549 cells treated by docetaxel.