中文摘要：

目的 对屎肠球菌进行全转录组测序,对比空间环境作用后菌株的基因表达差异。方法 提取菌株总RNA并进行测序文库构建,利用第二代高通量测序技术进行测序,对原始测序数据进行过滤、组装、注释和分析,并通过比对地面对照菌株的转录组分析空间飞行后屎肠球菌株的基因表达差异。结果 空间环境作用于屎肠球菌后,大量基因表达出现差异,包括1 036个基因表达下调,123个基因表达上调,基因本体功能和京都基因与基因组信号通路注释表明差异表达基因主要分布于生长和代谢相关通路。结论 屎肠球菌全转录组测序和分析明确,空间飞行后的屎肠球菌生长和代谢相关基因表达改变。

英文摘要：

Objective To compare the differences of gene expression of Enterococcus faecium exposed to space environment through whole transcriptome sequencing. Methods The total RNA of Enterococcus faecium was extracted and library was constructed followed by sequencing on HiSeq 2000. Then the raw datum of transcriptome was obtained, filtered, assembled and annotated. The difference of gene expression was analyzed by comparing the transcriptome of Enterococcus faecium after spaceflight with one on the ground. Results After spaceflight, amounts of genes expressed differently, including 1 036 down-regulated genes and 123 upregulated genes. The annotation of GO function and KEGG pathway revealed genes with different expression were mainly associated with bacteria growth and metabolism. Conclusion The whole transcriptome of Enterococcus faecium strain is sequenced and annotated clearly, which suggests that after spaceflight the expression of genes relating with growth and metabolism has changed.