中文摘要：

目的观察益气活血解毒方药中主要活性物质皂苷、黄酮、多糖等组合对脑缺血再灌注损伤的作用及其机制。方法SD大鼠随机分为假手术组，脑缺血再灌注模型组，皂苷组，黄酮组，多糖组，活性物质组合组，尼莫地平组，每组10只。线栓法制备大脑中动脉局灶性栓塞模型，观察各组对大鼠神经缺损功能的恢复，脑梗死体积，脑指数、脑含水量，缺血侧脑组织超氧化物歧化酶活力、丙二醛含量，白细胞介素-1β、白细胞介素-α和肿瘤坏死因子-α[等炎症因子的含量，Bcl-2和Bax蛋白表达，尼氏小体数的影响。结果益气活血解毒方药主要活性物质皂苷、黄酮、多糖活性物质组合配伍能显著地减轻中动脉局灶性栓塞模型大鼠的神经功能缺损症状，促进神经功能缺损症状恢复；缩小大鼠中动脉局灶性栓塞后脑组织梗死体积，降低脑梗死体积百分率；减轻脑水肿，降低脑指数和脑含水量；提高缺血再灌注后大鼠脑组织中超氧化物歧化酶活性、降低丙二醛含量；降低缺血侧脑组织白细胞介素-1β、白细胞介素-α和肿瘤坏死因子-α等炎症因子的含量；上调Bcl-2蛋白、下调Bax蛋白表达，调节两者比例；抑制尼氏小体数的减少。结论益气活血解毒方药中主要活性物质组合对大鼠脑缺血再灌注引起的损伤具有明显的保护作用。

英文摘要：

cine on cerebral ischemia and reperfusion injury. METHODS SD rats were randomly divided into sham-operation group, model group, saponin group ,flavonoids group, polysaccharide group, combination group of saponin, flavonoids and polysaccharide, and nimodipine group. Using focal middle cerebral artery embolization（MCAO） model,the symptoms of neurological deficit score were observed ;the infarct volume, brain index, brain water content, SOD activity, the contents of MDA, IL-1β, IL-6and TNF-αof ischemic brain tissue, the expressions of Bcl-2 and Bax proteins, and the quantity of nissl Body were determined. RESULTS The combination with saponin, flavonoids and polysaccharide,the main active part of Yiqi Huoxue Jiedu Recipe, could significantly reduce the symptoms of neurological deficits, promote the recovery of neurological deficits, narrow infarct volume of brain tissue, reduce the percentage of infarct volume, brain index and brain water, raise SOD activity, reduce the contents of MDA,IL-1β, IL-6 and TNF-αof ischemic brain tissue. It also increased Bcl- 2 protein, reduced Bax expression and reperfusion significantly. The reduction or disappearance of nissl body was inhibited. CONCLUSION The combination with saponin,flavonoids and polysaccharide has significant protective effects on ischemic brain injury.