本研究利用阴离子型芘衍生物8-羟基芘-1,3,6-三磺酸三钠盐(HPTS),建立了一种快速、可视化的对硝基苯胺(PNA)荧光检测方法。检测原理主要是基于PNA通过非共价作用而导致的HPTS的荧光淬灭。通过测量加入PNA后HPTS在512 nm处荧光强度的变化,可以实现PNA的荧光检测。本方法的线性范围为10~120μmol/L,检出限(3σ/s)为4.6μmol/L。对本方法的抗干扰性以及方法的实际应用性进行了考察。结果表明,本方法具有低成本、高灵敏度、高选择性、操作简便等优点。
p-Nitroaniline(PNA) was one kind of highly toxic aromatic amine and becomes an environmental pollutant in recent years.Here we reported the construction of a fluorescent sensor based on an anionic pyrene,8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt(HPTS),for the rapid and visual detection of PNA in aqueous media.The fluorescence quenching of HPTS caused by the presence of PNA was through non-covalent interactions.A good linear relationship between fluorescent intensity of HPTS at 512 nm was obtained in the range of 10-120 μmol/L.The detection limit(3σ) of this approach was 4.6 μmol/L.The results showed that the method was suitable for rapid detection of PNA in real samples with good sensitivity,selectivity,antiinterference,low cost and easy operation.