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中文摘要：
目的：探讨大鼠角膜层间植入微粒体诱导新生血管模型的制作技巧和特点。 方法：9只SD大鼠手术显徽镜下做角膜基质微囊袋并植入含有VEGF的缓释微粒体。术后1、3、5、7d采用裂隙灯显徽镜观察角膜新生血管的生长情况。 结果：大鼠角膜微囊袋内植入微粒体后1d仅见角膜缘血管网充血扩张，未见新生血管生长；3d可见毛刷状新生血管自角膜缘伸入角膜，面积为（2．23±0．59）mm^2；5d新生血管垂直于角膜缘向微粒体方向柬状延伸，并达到徽粒体下缘部位，面积为（6．81±1．35）mm^2；7d新生血管继续向前生长，达到并超过微粒体下缘，部分新生血管顶端互相吻合成袢状，面积为（8．92±1．79）mm^2。其中，5d和7d新生血管面积比较无显著性差异。所有术眼均未见前房积血、角膜溃疡、微粒体脱出等并发症。 结论：大鼠角膜层问植入微粒体诱导的新生血管生长稳定、无并发症、适用于定量研究。
英文摘要：
AIM： To explore the skills and characteristics of corneal neovascular model in rat induced by micropocket assay. METHODS- Nine eyes of nine Sprague-Dawley rats were studied. Pellets made of vascular endothelial growth factor （VEGF）, poly-2-hydroxylethylmethacrylate and sucralfate were implanted into the corneal stroma no closer than l mm from the limbus. Biomicroscopic features of corneal neovascular were observed on 1,3, 5, 7th day after the implantation. RESULTS： On day 1 after operation, the limbal vessels were dilated, with no angiogenesis appeared. On day 3, angiogenesis began to invade peri-cornea with a brush shape, the area of CNV was （2.23±0.59）mm^2. On day5, new vessels reached the lower margin of pellet densely, the area of CNVwas （6.81 ±1.35）mm^2, On day 7, new vessels continued to elongate, parts of them extended as loops toward the pellet, the area of CNV was （8. 92±1.79） mm^2. Neither hyphema or other complications occurred. CONCLUSION： Corneal neovascular induced by micropocket assay in rat grows steadily, with no complication, and is suitable for quantitative researches.
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