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中文摘要：
目的研究发状分裂相关增强子-1（HESR-1）基因在血管形成和维持中的作用。方法体外培养人脐静脉内皮细胞（HUVEC），构建PcDNA3．1＋HESR-1重组质粒、RNA干扰用pSIREN＋HESR-1质粒。脂质体将这两个质粒分别转染到HUVEC，并以转染空白质粒体作为对照。在HUVEC内分别高表达HESR-1、下调HESR-1的表达，采用半定量逆转录-聚合酶链反应（RT—PCR）和免疫印迹法检测HUVEC内HESR-1不同表达状态时血管内皮生长因子第2受体（KDR）、激活素受体样激酶1（ALK-1）、血管生成因子1（Ang-1）表达的改变。结果在HUVEC内高表达HESR-1基因能下调KDR的表达，上调ALK-1、Ang-1的表达；当抑制HESR-1基因，KDR的表达上调，ALK-1、Ang-1的表达下调。结论HESR-1基因可调控HUVEC中KDR、ALK-1、Ang-1的表达，在血管的形成和维持中发挥关键作用。
英文摘要：
Objective To study the roles of hairy and enhancer of split related-1 （ HESR-1 ） in maintenance of the mature, quiescent vessel and angiogenesis. Methods Human umbilical vein endothelial cells（HUVEC） were cultured. The full-length coding sequence of HESR-1 was cloned into PcDNA3. 1 ＋ using standard protocols. HESR-1 specific siRNA was synthesized and cloned into the RNAi-Ready pSIREN- RetroQ ZsGreen Vector. The constructed PcDNA3.1 ＋ HESR-1 plasmid were transfected into HUVEC for the overexpression of HESR-1, and HESR-1-RNAi plasmid were transfected into HUVEC to silence the HESR-1 gene, the expression of KDR , ALK-1 and Ang-1 in HUVEC were analyzed by RT-PCR and Western blot. Results The expression of KDR was down-regulated and ALK-1 and Ang-1 were up-regulated in HUVEC with the overexpression of HESR-1 ; The expression of KDR was up-regulated and ALK-1 and Ang-1 were down-regulated the HESR-1 in HUVEC by RNAi. Conclusion HESR-1 may play an important role in maintenance of vessel in quiescent and control angiogenesis by the regulation of the expression of KDR ,ALK- 1 and Ang-1.
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