中文摘要：

应用微板发光毒性测试方法，测定了9种取代酚及其混合物对淡水发光菌——青海弧菌（Q67）的发光抑制百分率（即毒性指标），进而通过非线性最小二乘法对测定的剂量-效应曲线进行模拟，以等效应浓度比法设计混合物，借剂量加和（DA）与独立作用（IA）模型评估混合物的混合毒性。试验发现：9种取代酚对Q67的毒性大小（以pEC50表示）依次为：苯酚〈间甲苯酚〈邻氯苯酚〈对甲苯酚〈间硝基苯酚〈邻硝基苯酚〈对氯苯酚〈对硝基苯酚〈2，4-二氯苯酚；按等效应浓度比，如EC50、EC20、EC10、EC4和EC1构成的5种酚混合物的混合毒性，既可以用DA模型、也可用IA模型进行评估与预测。

英文摘要：

Toxicities of 9 substituted phenols and their mixtures to fresh water photobacteria, Vibrio-qinghaiensis sp. -Q67, are determined by micro-plate luminometry. Then a nonlinear least square is fitted to the observed dose-response curves. An equal-effect concentration ratio approach is used to construct the test mixtures whose joint toxicities are evaluated by the dose addition （DA） and independent action （IA） models. It is found that the toxicity order among 9 substituted phenols to Q67 according to their individual pECs0 （ phenol 〈 m-methylphenol 〈 o-chlorophenol 〈p-methylphenol 〈 m-nitrophenol 〈 o-nitrophenol 〈p-chlorophenol 〈p-nitrophenol 〈 2, 4-dichloroophenol） and the toxicities of 5 mixtures composed of the equal-effect concentrations （ EC50, EC20, EC10, EC4 and EC1 ） can be effectively assessed by DA or IA model.