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中文摘要：
目的： 应用酵母双杂交系统筛选血液中与人体生物钟蛋白Per1相互作用的新蛋白。方法： 以人Per1的basic HLH-PAS结构域为诱饵,在人血液cDNA文库中筛选与之相互作用的新蛋白.阳性克隆送测序后用生物信息学分析。结果： 酵母双杂交文库营养缺陷筛选得到114个阳性克隆,β-半乳糖苷酶检测报告基因获得46个蓝色克隆。通过测序和生物信息学分析,其中之一能与Per1相互作用的蛋白为LSMD1。结论 ：通过酵母双杂交系统证明Per1能够与LSMD1发生相互作用。
英文摘要：
Objective To find a new method for identifying interacting proteins of human Perl in the blood. Methods Using bHLH-PAS domain of hPerl as bait, a human blood cDNA library was screened and the proteins interacting with hPerl were searched. The positive clones were sequenced and analyzed by bioinformatics methods. Results Forty-six colonies were selected by the yeast two-hybrid system. LSMDI protein was obtained after verification. Conclusion It is proved that Perl can interact with LSMD1 by yeast two-hybrid system.
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