中文摘要：

目的：在H2O2胁迫下．研究角黄素增强成骨细胞抗自由基损伤的作用。方法：用H2O2作用M3T3-E1成骨细胞，建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、角黄素低剂量组（1×10^-8mol／L）、角黄紊中剂量组（1×10^-7mol／L）和角黄素高剂量组（1×10^-6mol／L）。测定不同处理组细胞活力、超氧化物歧化酶（superoxide dismutase，SOD）活性、活性自由氧（reactive oxygen species，ROS）含量、脂质过氧化物（lipid oxygen，LPO）含量，同时利用荧光偏振法测定细胞膜流动性。结果：模型组细胞活力、SOD活性、细胞膜流动性均比不同角黄素组有显著降低（P〈0．01），而ROS含量、LPO含量均比不同角黄素组有显著升高（P〈0．01）。结论：H2O2摄入会导致大鼠成骨细胞的氧化损伤，而角黄素可以预防或降低此类损伤对细胞的影响。

英文摘要：

Objective：To investigate the effects of canthaxanthin on the anti-oxidative damage function of osteoblast. Methods： M3T3-E1 osteoblasts were randomly divided into five groups, including control group,model group,canthaxanthin groups [low-dose（ 1×10^-8 mol/ L）,middle-dose （1 ×10^-7 mol/L）,high-dose （1×10^-6 mol/L）]. The activity of cells, superoxide dismutase （SOD）, the content of reactive oxygen species （ROS）, lipid oxygen （LPO） and membrane fluidity were tested and compared. Results：Compared with canthaxanthin groups, the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased （P 〈 0.01 ）. However, the content of ROS and LPO were significantly increased（P 〈 0.01 ）. Conclusion：H2O2 can induce oxidative damage of M3T3-E1 osteoblasts, but canthaxanthin can prevent and decrease its influence.