中文摘要：

三七总皂苷（PNS）属于达玛烷型三萜皂苷,是中国传统珍贵药材三七的主要活性成分,三七总皂苷在中枢神经系统、心脑血管系统、血液系统、免疫系统以及抗纤维化、抗衰老、抗肿瘤等方面均具有较好的生理活性。三七总皂苷是以达玛烯二醇为前体,在P450单加氧酶和糖基转移酶催化下形成。另外,三七总皂苷与植物甾醇共享前期代谢途径。该文对近年来国内外有关三七总皂苷的生物合成途径及关键酶基因的最新研究进展进行综述,并探讨了代谢工程在三七总皂苷生物合成中的应用前景。

英文摘要：

Panax notoginseng saponins（PNS）,which belongs to dammarane type triterpenoid saponins,are the main active components of Chinese traditional valuable medicinal herb Panax notoginseng.Studies have shown that PNS have good physiological activities in central nervous system,cardio-cerebrovascular system,blood system,immune system,anti-fibrosis,anti-aging,and anti-tumor.PNS are synthesized from dammarenediol under cyclization of P450 monooxygenase and glycosyltransferase.In addition,PNS share early biosynthesis steps with phytosterols.This article reviewed the recent advances of PNS biosynthesis,the cloning and characterization of genes encoding key enzymes in the pathway,and briefly discussed the application of metabolic engineering in PNS biosynthesis.