中文摘要：

组合生物合成技术作为一种合成＂非天然＂自然产物的新手段,已被成功应用并取得很大的进展。利用组合生物合成技术人工构建生物代谢途径并同源或异源表达目的产物已成为研究热点。近年来,该技术在研究萜类化合物和聚酮类化合物合成方面有所成果并日趋完善,已有成功在大肠杆菌中人工构建代谢途径产生聚酮类、萜类物质的报道。文章总结了该技术在青蒿素、紫杉醇等萜类物质及聚酮类化合物中的应用现状及研究进展。

英文摘要：

With the development of molecular biology, the combinatorial biosynthesis as a new method which synthesizes new ＂non- natural＂ natural products has been successfully used and has a lot of progress. It has become a hot topic that the desired product has been homologous or heterologous expression by use of combinatorial biosynthesis. An Escherichia coli or yeast system carrying artifi- cial biosynthetic pathways for production of plant-specific biologically active substances, such as arteannuin, taxol and flavonoids, is designed and expressed. In recent years, there has mainly research on terpenoids and polyketides and some results have been achieved. It has been reported that terpenoids and polyketides have been successfully produced by artificial biosynthetic pathways in Escherichia coll. A summdary about research process and present situation on artemisinin, paclitaxel of terpenoids and polyketides has been reviewed.