中文摘要：

目的：研究三七细胞悬浮培养过程中皂苷生物合成的动力学变化。方法：将三七愈伤组织进行悬浮培养40d，用HPLC检测细胞生长过程中皂苷的动态变化。结果：三七愈伤组织悬浮培养生物量增长缓慢，但检测到6种皂苷的含量随培养时间而变化，人参皂苷Rg1、Re、Rh1、Rb1初始培养值分别为3．63、2．34、0．42、0．24mg／g，培养25-30d后增至最高，分别为9．72、7．02、2．37、1．86mg/g，而Rh2、F1含量无明显变化，即三七愈伤组织生物合成过程中原人参三醇型皂苷（Rg1、Re和Rh1）增长显著。结论：三七愈伤组织培养25d，总皂苷含量达到最高20．58mg／g，主要为原人参三醇型皂苷。

英文摘要：

Objective：To study the dynamics of ginsenoside biosynthesis in suspension culture of Panax notoginseng. Methods ：The ealli of Panax notoginseng were suspended in liquid medium for 40 d, and the changes of ginsenosides were determined by HPLC method. Results： Cell growth was slow in suspension culture, but the contents of six ginsenosides varied with different culture time. The contents of four ginsenosides RgI ,Re,Rh1 and RbI showed the increases from about 3.63 to 9. 72 mg/g,2. 34 to 7.02 mg/g,0. 42 to 2. 37 mg/g and 0. 24 to 1.86 mg/g,respectively during 25 and 30 days of the culture. Rh2 and F1 had no obvious change. Such results indicated that the protopanaxatriol saponions（ Rg~ ,Re and Rh1 ）increased significantly during the growth phase. Conclusion：When the callus is cultured for 25 days, the maximum yield of total saponins content is up to 20. 58 mg/g, mainly of the protopanaxatriol saponins.