中文摘要：

目的 从新发现的新疆民丰县黑热病疫区分离利什曼原虫。方法 用镜检法调查白蛉自然感染利什曼原虫状况,以接种敏感动物和培养基方法分离利什曼原虫。结果 捕捉白蛉282只,检查173只吴氏白蛉（♀）,查出感染前鞭毛体白蛉9只,分别培养接种9只灰仓鼠,其中2只接种第二天死亡;分离出2株利什曼原虫,灰仓鼠出现典型的内脏利什曼病症状。结论 分离自吴氏白蛉的利什曼原虫致使灰仓鼠表现出典型的内脏利什曼病症状,分离到的利什曼原虫对研究塔里木盆地的利什曼原虫进化具有重要的意义。

英文摘要：

Objective To isolate leishmania from new kala-azar foci in Minfeng County of Xinjiang, China. Methods Natural infection status of leishmania in sandflies from kala-azar foci in Minfeng County was surveyed with microscopy method, and leishmania isolated by inoculating sensitive animals and medium. Results Out of 282 sandflies captured, 9 were found infection with promastigotes in 173 female ofPhlebotomus wui. The infected sandflies were cultured and inoculated in- to grey hamster （C. rnigratorius） respectively and 2 strains of leishmania isolated from the hamsters, showing typical symp- toms of visceral leishmaniasis. Conclusions The isolated strains of leishmania from P wui cause grey hamsters to show typical symptoms of visceral leishmaniasis. The isolation of leishmania is of an important significance for studying leishmania evolution in the Tarim Basin.