中文摘要：

目的探讨microRNA（miRNA）在肾透明细胞癌组织中的表达及其临床意义。方法用实时荧光定量PCR技术检测20例肾透明细胞癌患者癌组织与癌旁组织中miRNA-20b、-21、-34a、-210、-141、-200c、200b、-514、-532-5p的表达变化。结果与癌旁组织相比,肾透明细胞癌患者癌组织中miRNA-21、-34a、-210呈高表达（P〈0.05）,miRNA-141、-200c呈低表达（P〈0.05）,miRNA-20b、-200b、-514、-532-5p则无差异。miRNA-34a随着肾癌病理分级的增高而增高。结论miRNA-21、-34a、-210、mir-141、-200c在肾透明细胞癌患者癌组织中表达存在差异,可能在肾透明细胞癌中起重要作用。

英文摘要：

Objective To study miRNAs expression in renal clear cell carcinoma and its clinical significance.Methods The expression levels of miRNA-20b,miRNA-21,miRNA-34a,miRNA-210,miRNA-141,miRNA-200c,miRNA-200b,miRNA-514 and miRNA-532-5p in renal clear cell carcinoma tissues and their corresponding adjacent tissues were analyzed with qRT-PCR;their relationships with clinical pathological features were analyzed with statistical method.Results Compared with adjacent tissue,miRNA-21,miRNA-34a,and miRNA-210 were high-expressed in renal cell carcinoma while miRNA-141 and miRNA-200c were low-expressed（P0.05）,the expression levels of miRNA-20b,miRNA-200b,miRNA-514 and miRNA-532-5p kept unchanged.The expression level of miRNA-34a increased with the tumor grade.Conclusion miRNA-21,miRNA-34a,miRNA-210,miRNA-141 and miRNA-200c were differentially expressed in renal clear cell carcinoma;they may be used as potential diagnostic and therapeutic targets for renal cell carcinoma. To study miRNAs expression in renal clear cell carcinoma and its clinical significance.Methods The expression levels of miRNA-20b,miRNA-21,miRNA-34a,miRNA-210,miRNA-141,miRNA-200c,miRNA-200b,miRNA-514 and miRNA-532-5p in renal clear cell carcinoma tissues and their corresponding adjacent tissues were analyzed with qRT-PCR;their relationships with clinical pathological features were analyzed with statistical method.Results Compared with adjacent tissue,miRNA-21,miRNA-34a,and miRNA-210 were high-expressed in renal cell carcinoma while miRNA-141 and miRNA-200c were low-expressed（P0.05）,the expression levels of miRNA-20b,miRNA-200b,miRNA-514 and miRNA-532-5p kept unchanged.The expression level of miRNA-34a increased with the tumor grade.Conclusion miRNA-21,miRNA-34a,miRNA-210,miRNA-141 and miRNA-200c were differentially expressed in renal clear cell carcinoma;they may be used as potential diagnostic and therapeutic targets for renal cell carcinoma.