中文摘要：

目的：研究ITSN1-S蛋白在调节恶性胶质瘤细胞增殖与凋亡方面的作用，并进一步探讨其相关的分子机制。方法：利用小RNA干扰技术抑制体外培养的人胶质母细胞瘤细胞LN-229中ITSN1-S蛋白的表达，并应用MTT、流式细胞术、TUNEL染色等方法检测胶质母细胞瘪细胞的增殖与凋亡情况，进而应用Western blotting法检测与细胞凋亡密切相关的蛋白（Bad、Bcl-2）的表达情况。结果：与对照组相比，转染组细胞的增殖受到明显抑制（P〈0．05），凋亡显著增加（P〈0．05），并且促凋亡蛋白Bad的表达增加，抗凋亡蛋白Bcl-2的表达下降。结论，ITSN1-S参与调节胶质母细胞瘤细胞的增殖与凋亡，该作用可能与其调节凋亡相关蛋白的表达有关。

英文摘要：

Objective： To discuss the biological significance of ITSNI-S in regulating proliferation and apoptosis of glioblastoma cells and to further explore the possible molecular mechanisms. Methods： Small interfering RNA （siRNA） technology was applied to inhibit the expression of ITSN1-S in the glioma cell line LN-229. MTT assay, flow cytometry and TUNEL assay were performed to determine cell proliferation and apoptosis. Western blotting was used to detect the expression of apoptosis-related proteins, Bad and Bcl-2. Results： Compared with the control group, there was apparent inhibition of cell proliferation in the transfected group, with an increase of apoptosis. The expression of anti-apoptotic protein Bcl-2 was down-regulated while the expression of apoptosis-promoter protein Bad was up-regulated. Conclusion： ITSN1-S is involved in regulating the proliferation and apoptosis of glioma cells by regulating the expression of the apoptosis-related proteins.