以‘豫农982’(1BL/1RS易位)和wheatear(7DL.7Ag易位)杂交后代的900个F2群体及其F2:3家系为实验材料,对F2群体进行1BL/1RS易位和7DL.Ag易位类型分子检测,调查分析F2群体及F2:3家系的农艺、产量性状(Fz群体的农艺性状仅作参考,重点分析F2:3家系的性状),并探讨1BL/1RS易位和7DL.7Ag易位对小麦品质的影响。结果表明:(1)STS标记Lr19_130与SSR引物Xgwm428联合使用可作为共显性标记鉴定纯合7DL.7Ag易位与杂合7DL-7Ag易位,完善了7DL.7Ag易位的分子检测方法。(2)在农艺和产量性状方面,1BL/1RS易位可显著降低株高,有助于提高穗粒数和小穗数;7DIL.7Ag易位在籽粒千粒重和产量上有显著的正向作用,但7DL.7Ag易位的穗粒数显著低于非7DL.7Ag易位且有延迟小麦成熟和增加株高的趋势;1BL/1RS和7DL.TAg双重易位可同时提升小穗数和千粒重,但穗粒数减少。(3)在品质性状方面,1BL/1RS易位主要影响沉淀值、稳定时间、弱化度、最大拉伸阻力、延伸度等主要反映蛋白质质量的性状,使面筋质量显著降低,而对蛋白质含量、湿面筋含量和吸水率等主要反映蛋白质数量的性状影响较小;7DL.7Ag易位显著提高沉淀值和面团拉伸品质参数,对小麦粉质参数和糊化参数贡献不大。由此推测,将7DL.TAg易位应用于小麦品种选育,有望突破产量瓶颈并可较好地提升品质。
The 1BL/1RS translocation and 7DL. TAg translocation have been widely used by wheat breeders to enhance agronomic performance and disease resistance, In the past decades, the introduction and applica- tion of 1BL/1RS translocation significantly increased yield potentials and adaptability in China;now it still plays an important role in wheat breeding and production. Currently, the positive effects of 7DL. 7Ag trans- location in terms of increasing the yield have caused attention of wheat breeders over the world. The objec- tive of this study was to analysis the effects of 1BL/1RS and 7DL. TAg chromosome translocations on the main agronomic,yield and quality traits in wheat. A total of 900 F2 population and F2:3 family derived fromthe cross 'Yunong 982' (1BL/1RS translocation)/wheatear(7DL. 7Ag translocation) were planted in Yan- gling of Shaanxi Province during 2009-2011. This study identified the chromosome translocation types of F2 population using SSR and STS. In addition,a total of 15 traits of the two parents,F2 population and F2: 3 family, such as plant height, spike length, grain number per spike, thousand-grain weight, grain weight per spike and days from emerge to maturity,were investigated (this study took the agronomic traits of F2 popu- lation as a reference,the main agronomic traits of F2:3 family were this study's key point). The F-test and multiple comparisons of the agronomic traits of no-translocation lines,homozygous 1BL/IRS translocation lines,homozygous 7DL. 7Ag translocation lines and Double Translocation Lines (1BL/1RS,7DL. 7Ag) in F2,3 family were done using the SASS. 2 System. At the same time,the effects of 1BL/1RS translocation and 7DL. 7Ag translocation on wheat quality were tested. The results showed that. STS Lr19_130 ker and Xgwm428 marker were identified,which could effectively distinguish the heterozygous 7DL. TAg transloca- tion from homozygous 7DL. 7Ag translocation when they were used together. In terms of agronomic traits and yield traits,a significa