为了探究进化模型对DNA条形码分类的影响,本研究以雾灵山夜蛾科44个种的标本为材料,获得COI基因序列。使用邻接法(neighbor-joining)、最大简约法(maximum parsimony)、最大似然法(maximum likelihood)以及贝叶斯法(Bayesianinference)构建系统发育树,并且对邻接法的12种模型、最大似然法的7种模型、贝叶斯法的2种模型进行模型成功率的评估。结果表明,邻接法的12种模型成功率相差不大,较稳定;最大似然法及贝叶斯法的不同模型成功率存在明显差异,不稳定;最大简约法不基于模型,成功率比较稳定。邻接法及最大似然法共有6种相同的模型,这6种模型在不同的方法中成功率存在差异。此外,分子数据中存在单个物种仅有一条序列的情况,显著降低了模型成功率,表明在DNA条形码研究中,每个物种需要有多个样本。
The effects of evolution models on success rates of species identification via DNA barcoding were evaluated based on specimens of 44 Noctuidae species collected from Wuling Mountain, Hebei, northern China. Different phylogenetic tree reconstruction methods, including the neighbor-joining, the maximum parsimony, the maximum likelihood and the Bayesian inference, were used to reconstruct phylogenetic trees based on the COI gene sequences of these species, and success rates of different models ( 12 models for the neighbor-joining, 7 models for the maximum likelihood method, and 2 models for the Bayesian inference ) were evaluated systematically. The results showed that there were no significant differences in success rates of species identification among 12 different models for the neighbor- joining method, but significant differences in success rates were found among different evolution models for both maximum likelihood and Bayesian methods. As a comparison, the maximum parsimony method which is not based on evolution models obtained stable success rates of species identification. The 6 evolutionary models shared by the neighbor-joining and maximum likelihood methods obtained different success rates between these two methods. In addition, we found that singletons in the database largely reduced success rates of species identification, suggesting that deep intraspecific sampling be required in the future studies of DNA barcoding.