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中文摘要：
目的：揭示同一GAP产地同一批次的鲜蕺菜植株间的蛋白质多样性,为蕺菜＂节点代谢网络＂的构建奠定基础。方法：采用3种方法从3个角度对蕺菜植株间的蛋白质进行多样性研究,即采用Ramagli改进的Bradford法、SDS-PAGE凝胶电泳法和双波长薄层扫描法分别研究其总蛋白含量多样性、蛋白种类差异性及各种类的含量变异性。结果：53株蕺菜蛋白条带分子量大多集中在6.5~97.2 kDa范围内,蛋白种类差异性不明显,主要表现为条带颜色深浅不一;蛋白电泳条带扫描图的总量统计矩零阶矩（AUCT）、一阶矩（MCRTT）和二阶矩（VCRTT）的RSD分别为40.92%、6.01%和18.57%。结论：同一GAP产地不同植株鲜蕺菜个体间蛋白质具有丰富的多样性,为后续关键蛋白酶的寻找、＂节点代谢网络＂的构建奠定基础,也进一步拓展了中药质量的稳定性研究。
英文摘要：
Objective： To reveal protein diversity between the same batch of fresh Houttuynia cordata in the same GAP base,and to lay the foundation construction for＂node metabolic network＂. Methods： Three methods including the Ramagli improved Bradford law, SDS-PAGE gel electrophoresis method and double wavelength thin-layer scanning method were used to study the total protein content diversity,protein species diversity and various kinds of content variability. Results： The molecular weight of 53 plant protein mostly concentrated in the range of 6. 5 ~ 97. 2 kDa,the species diversity was not obvious with main performance for banding color shades; The RSD of zero moment（ AUCT）,first moment（ MCRTT） and second moment（ VCRTT） in protein electrophoresis banding was 40. 92%, 6. 01% and 18. 57%,respectively. Conclusion： There is rich diversity in different Houttuynia cordata plant in the same GAP base, which provides basis for the foundation of subsequent key protease search,＂node metabolic network＂construction,and study on the Chinese medicine quality stability.
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