中文摘要：

目的：研究凋亡抑制蛋白c-FLIP对肝癌细胞自噬及恶性生物学行为的影响。方法：在低糖条件下通过干扰c-FLIP的表达,明确c-FLIP对低糖诱导肝癌细胞自噬的影响。Transwell、细胞划痕、增殖实验及裸鼠荷瘤等方法分析肝癌细胞恶性表型。结果：干涉c-FLIP后,肝癌细胞自噬水平明显上升;c-FLIP干涉组肝癌细胞增殖、侵袭、运动能力均降低,差异具有统计学意义;与对照组相比,c-FLIP干涉组荷瘤裸鼠存活率上升。结论：c-FLIP抑制低糖诱导的肝癌细胞自噬发生,促进肝癌的增殖和侵袭能力,提示c-FLIP可能通过调节肝癌细胞自噬在肝癌的发生发展过程中发挥着重要作用。

英文摘要：

Objective： To investigate the effect of cellular caspase 8（ FLICE）- like inhibitory protein（ c- FLIP）on the autophagy activity and biological characteristics in Hep G2 cells. Methods： Imitating the low carbohydrates environment of hepatic carcinoma,we inhibited the expression of c- FLIP to set a treatment group and the other one was as control. To observe the expression of autophagy associated protein LC3 and p62,the formation of autophagosome by immunofluorescence and transmission electron microscope,detect the malignancy of hepatocyte Hep G2 by the transwell,wound healing,and cell proliferation assay. We also analyzed the survival rate of nude mice to evaluate the malignancy of the two groups. Results： The expression of LC3 and p62 and the number of autophagosome increased obviously over time after suppressing c- FLIP. The ability of proliferation,invasive and migration of the treatment group decreased severally. The survival rate of nude mice in treatment group was higher than the control（ P〈0. 05）. Conclusion： The expression of c- FLIP could apparently suppress the autophagy activity and efficiently enhance the malignancy of Hep G2,indicating that c- FLIP has an important role in the occurrence and development of hepatoma.