【目的】了解引诱物对白纹伊蚊Aedes albopictus行为及触角电生理影响,以揭示白纹伊蚊吸血与传病的行为机制。【方法】采用行为学和触角电位学的方法分别测定了L-乳酸、氨、1-辛烯-3-醇对白纹伊蚊的行为反应和触角电位强度变化,并分析了几种引诱物对白纹伊蚊活性的影响及其规律。【结果】氨、L-乳酸和1-辛烯-3-醇3种引诱物的引诱效果和触角电位变化趋势相同,随着引诱物浓度的增加,对白纹伊蚊的引诱效果和触角电位强度也相应增强。同等浓度下氨对白纹伊蚊的引诱效果较另外两种引诱化合物更显著,且1-辛烯-3-醇的引诱效果相比L哥L酸要更强。当引诱化合物浓度在1mg/L以上时,白纹伊蚊触角电位反应增幅随着浓度增大也逐渐增大;在引诱化合物浓度达1000mg/L时,白纹伊蚊对氨的触角电位反应最强烈。【结论】行为学和触角电位的研究手段相结合能更为全面地反映和评价不同引诱物对蚊虫的引诱效果。
[Objectives] To investigate the influence of attractants on the behavior and electroantennograms of Aedes albopictus and thereby reveal the behavioral mechanisms underlying blood-feeding behavior in this species. [Methods] Behavioral observation and electroantermography (EAG) were used to test the effects of ammonia, L-lactic acid and l-octene-3-alcohol on the behavior and physiology ofA. albopictus. [Results] The number of insects attracted, and EAG intensity induced, by the three attractants increased with concentration. At the same concentrations, ammonia was the most attractive compound, followed by l-oetene-3-alcohol and L-lactic acid. At concentrations above 1 mg/L, EAG intensity gradually increased with concentration. At concentrations of up to 1 000 mg/L, ammonia elicited the strongest reaction. [Conclusion] Combining behavioral observation with EAG is a better way of determining the relative attractiveness of different substances to mosquitoes.