中文摘要：

目的比较老年学习记忆减退大鼠接受艾灸治疗后海马干细胞相关基因的变化,寻找与艾灸有关的海马干细胞相关的基因靶点。方法将经行为学筛选后的老年SD大鼠随机分为对照组、模型组和艾灸组。选用“百会”、“命门”穴位艾灸治疗,每次10min,每日1次,10次为1疗程,6个疗程后取海马组织以干细胞基因芯片筛选与艾灸有关的干细胞相关基因。结果在258条基因位点中,模型组与正常组比较,17条基因表达上调,36条基因表达下调;艾灸组与模型组比较,40条基因表达上调,43条基因表达下调。结论艾灸对老年学习记忆减退大鼠海马干细胞相关基因的影响是多方面的,重点靶点可能主要在于Fgf、Tgf、Wnt和Sox家族。

英文摘要：

Objective To detect related gene changes of hippocampus stem cells in aged cognitive decline rats treated by moxibustion and to screen the stem cells gene targets. Methods The aged SD rats screened by ethology were randomly divided into control, model and moxibustion （Baihui, life care moxibustion, 10 min every time, once a day, 10 times as a course of treatment） groups. The stem cell biochip was used to seek for the changed stem cell related genes in hippocampus after 6 courses of treatment. Results Of 258 gene locuses, there were 17 upregulating genes and 36 down-regulating genes in model group comapring to those in control group; 40 upregnlating genes and 43 down-regulating genes in moxibustion group comparing to those in model group. Conclusions The moxibustion therapy can regulate many kinds of genes expression in learning and memory defict aged rats hippocampus, especially Fgf, Tgf, Wnt and Sox family.