中文摘要：

免疫分析（Immunoassay，IA）是利用抗原（Antigen，Ag）和抗体（Antibody，Ab）之间的特异性反应来选择性识别和测定作为抗原或抗体的待测物．毛细管电泳免疫化学发光分析（Capillary electrophoresis immunoassay chemiluminescence detection，CEIACL）结合了CE的高分离效率、IA的高特异性及CL检测的高灵敏度的优点，同时还具有样品耗量少和仪器简单等特点．Mangru和Harrison首次利用辣根过氧化物酶（Horseradish peroxidase，HRP）对鲁米诺-过氧化氢的催化作用，应用芯片毛细管电泳分离化学发光法检测了HRP标记的羊抗鼠免疫球蛋白（IgG）的F（ab’）2片段和鼠IgG的免疫反应产物．Tsukagoshi等利用CEIACL检测了鼠IgG和HRP标记的抗鼠IgG免疫反应产物．本组利用CEIACL方法检测了鼠血管平滑肌细胞中骨形成蛋白-2，检出限达到6．24×10^-12mol／L．最近，Wang和Ren也开展了CEIACL研究，并实现了人血清中CA125的定量分析．

英文摘要：

A novel detection method of a tumor marker alpha-fetoprotein （AFP） in human sera via capillary electrophoresis immunoassay coupled with chemiluminescence detection （CEIACL） was established. After a simple non-competitive immunoassay procedure, seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by p-iodophenol. The detection limit （S/N = 3） of AFP is 0. 85 μg/L. The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera, which coincides with the results detected by Beckman automatic CL analyzer-access IA system.