中文摘要：

由 lipopolysaccharide (LPS ) 在单核白血球和导出单核白血球的巨噬细胞在这试验像使用费的受体表示模式刺激被检验。颈部的静脉的血从四头日本小牛被收集，并且外部血 mononuclear 房间(PBMC ) 被孤立。房间直接被使用由磁性的房间排序收集单核白血球或为 7 天到有教养在 Repcell 收集导出单核白血球的巨噬细胞。然后，我们为 24 h 在 LPS 刺激以后在单核白血球和导出单核白血球的巨噬细胞分析了 TLR 和 cytokines 的 mRNA 表示模式。单核白血球和导出单核白血球的巨噬细胞的 LPS 刺激为 TNF- ， IL-6 和 IL-8 导致了 mRNA 抄本的层次的增加。而且， TNF-6 和 IL-6 mRNA 表情被 LPS 刺激显著地在导出单核白血球的巨噬细胞扩充。TLR mRNA 表情在单核白血球的 LPS 刺激以后是未改变的，当在导出单核白血球的巨噬细胞的 TLR mRNA 表情是复杂的时。TLR1， 3， 5， 8 和 10 显著地在 LPS 刺激以后被减少，在 TLR2 的 mRNA 表情没有差别， 4， 6 和 7 在控制和 LPS 刺激的组之间。而且， TLR9 的表示都没被发现。作为介绍房间的抗原，单核白血球和导出单核白血球的巨噬细胞对 LPS 不同地作出回应，因此他们可以在天生的免疫系统有不同功能。

英文摘要：

In this experiment Toll-like receptor expression pattern in monocytes and monocyte-derived macrophages by lipopolysaccharide （LPS） stimulation was examined. Jugular venous blood was collected from four Japanese calves, and the peripheral blood mononuclear cells （PBMCs） were isolated. The cells were directly used for collecting monocytes by magnetic cell sorting or cultured for 7 days to collect monocyte-derived macrophages in Repcell. Then we analyzed the mRNA expression pattern of TLRs and cytokines in monocytes and monocyte-derived macrophages after LPS stimulation for 24 h. LPS stimulation of both monocytes and monocyte-derived macrophages resulted in an increase in the levels of mRNA transcripts for TNF-α IL-6 and IL-8. Moreover, TNF-α and IL-6 mRNA expressions were significantly augmented by LPS stimulation in monocyte-derived macrophages. TLRs mRNA expressions were unchanged after LPS stimulation of monocytes, while TLRs mRNA expressions in monocyte-derived macrophages were complicated. TLR1, 3, 5, 8 and 10 were significantly decreased after LPS stimulation and there were no differences in the mRNA expressions of TLR2, 4, 6 and 7 between the groups of control and LPS stimulation. Besides, no expression of TLR9 was found. As antigen presenting cells, monocytes and monocyte-derived macrophages respond differently to LPS, so they may have different functions in the innate immune system.